Sample
Serum, plasma, tissue homogenates and other biological fluids.
Intended Use
In vitro quantitative determination of cTnT/TNNT2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. ELISA Microplate: 8×12, 2-8°C/-20°C
2. Lyophilized Standard: 2 vial, 2-8°C/-20°C
3. Sample Dilution Buffer: 20 mL, 2-8°C
4. Biotin-labeled Antibody (100×): 1 vial, 120 μL, 2-8°C (Avoid Direct Light)
5. Antibody Dilution Buffer: 10 mL, 2-8°C
6. HRP-Streptavidin Conjugate (SABC) (100×): 120 μL, 2-8°C (Avoid Direct Light)
7. SABC Dilution Buffer: 10 mL, 2-8°C
8. TMB Substrate: 10 mL, 2-8°C (Avoid Direct Light)
9. Stop Solution: 10 mL, 2-8°C
10. Wash Buffer (25×): 30 mL, 2-8°C
11. Plate Sealer: 5 pieces
12. Product Description: 1 copy
Storage
2-8°C (for sealed box), please do not freeze! See kit label for expiry date.
Detection Range
15.625-1000 pg/ml
General Description
Uniprot ID: P45379
Troponin T is one of three troponin isoforms found in the tropomyosin-troponin complex. This complex is responsible for the calcium sensitivity of the contractile apparatus in the muscle. Cardiac Troponin T is used as a biological marker for cardiomyocytes and its level in serum is frequently used as an indicator of myocardial cell injury.
Standard Curve
This product has been tested by Quality Control Department and meets performance specifications mentioned in the manual. (The humidity in the laboratory is 20%-60%, and the temperature is 18°C - 25°C. TMB was balanced to 37°C before color development, and incubated at 37°C for 15 minutes in the dark after adding the enzyme label plate holes.) The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.
General Notes
Cardiac Marker ELISA Kits
Citations
Publication ()
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COA
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