This ELISA allows rapid and quantitative measurement of rat anti-KLH IgM levels in serum or plasma samples.
Contents of Kit
No.
Components
Size
Storage Conditions
1
KLH coated 96-well plate
12 × 8 wells
2-8°C
2
Enzyme Conjugate Reagent
11 ml
2-8°C
3
Reference standard
1 vial (lyophilized)
2-8°C
4
20× Wash Solution
50 ml
2-8°C
5
10× Diluent
25 ml
2-8°C
6
TMB Reagent (One-Step)
11 ml
2-8°C
7
Stop Solution (1N HCl)
11 ml
2-8°C
Storage
The kit should be stored at 4°C, and the microtiter plate should be kept in a sealed bag with desiccant to minimize exposure to damp air. Test kits will remain stable for six months from the date of purchase provided that the components are stored as described above.
General Description
Recent studies have demonstrated that suppression of anti-KLH (keyhole limpet hemocyanin) IgM levels by therapeutic agents serves as a useful indicator of immunosupression.
Standard Curve
A typical standard curve with optical density readings at 450nm on the Y-axis against anti-KLH IgM concentrations on the X-axis is shown below. This curve is for the purpose of illustration only and should not be used to calculate unknowns.
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