Monkey IgM ELISA Kit

Sample

Serum, Plasma, Urine

Species Reactivity

Monkey

Detection Method

sELISA

Intended Use

The lgM test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring lgM in monkey biological samples. If the ELISA is to be used outside the intended use, the user may need to optimize for said use.

Contents of Kit

The expiration date for the kit and its components is stated on the box label. All components should bestable up to the expiration date if stored and used per this kit protocol insert.
1. ELISA Micro Plate, antibody coated: One plate of 12 removable 8 well strips, antibody coated. Ready to use as supplied. 2-8°C, In sealed foil bag with desiccant. Stability: With proper storage the plate strips are stable until the expiration date.
2. Enzyme Conjugated Detection Antibody: One vial of 150μL of 100× Horseradish Peroxidase Conjugated antibody in a stabilizing buffer. Preparation: Dilute 1/100 immediately prior to use. 2-8°C in the dark. Stability: The working conjugate solution should be diluted immediately prior to use. The 100× conjugate is stable until the expiration date.
3. Calibrator: One vial of calibrator. The calibrator used in this kit is of human origin. The use of a human standard allows export of this kit. Preparation: Refer to the Certificate of Analysis (CoA). 2-8°C for lyophilized
calibrator. Aliquoted and frozen if reconstituted. Avoid multiple freeze-thaw cycles. Stability: The working standard solutions should be prepared immediately prior to use.
4. Diluent Concentrate: One 50 mL bottle of 5× diluent buffer. Preparation: Dilute 1/5 to make 1× working solution. 2-8°C for both 1× working solution and 5× concentrate. Stability: The 1× working solution is stable for at least one week from the date of preparation. The 5× concentrate is stable until the expiration date.
5. Wash Solution Concentrate: One 50 mL bottle of 20× wash solution. Preparation: Dilute 1/20 to make 1× working solution. 2-8°C for both 1× working solution and 20× concentrate. Stability: The 1× working solution is stable for at least one week fromthe date of preparation, The 20× concentrate is stable until the expiration date.
6. Chromogen-Substrate Solution: One bottle of 12 mL 3.3'.5.5-tetramethylbenzidine(TMB) and hydrogenperoxide in citnc acidbuffer at pH 3.3. Ready to use as supplied. 2-8°C in the dark. Stability: Protect from light. The Substrate Solution is stable until the expiration date.
7. STOP Solution: WARNING: Avoid Contact with Skin. One 12 mL bottle of 0.3 M sulfuric acid. Ready to use as supplied. 2-8°C. Stability: The Stop Solution is stable untilthe expiration date

Storage

2-8°C

Standard Curve