Serum, plasma, cell culture supernatant and other biological samples
Species Reactivity
Human
Detection Method
Sandwich-ELISA
Intended Use
The kit is intended for in vitro quantitative determination of IgM concentrations in serum, plasma, cell culture supernatant and other biological samples.
Contents of Kit
No.
Components
Size
Storage Conditions
1
ELISA Microplate(Dismountable)
96T (8×12)
2-8°C/-20°C
2
Lyophilized Standard
2 vials
2-8°C/-20°C
3
HRP-labeled Antibody (Concentrated, 100×)
120ul
2-8°C (Avoid Direct Light)
4
TMB Substrate
10ml
2-8°C (Avoid Direct Light)
5
Sample Dilution Buffer
20ml
2-8°C
6
Antibody Dilution Buffer
10ml
2-8°C
7
Stop Solution
10ml
2-8°C
8
Wash Buffer(25×)
30ml
2-8°C
9
Plate Sealer
5 pieces
Storage
2-8°C for 6 months
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Detection Range
1.563-100ng/ml
Sensitivity
< 0.938ng/ml
Standard Curve
This product has been tested by Quality Control Department and meets performance specifications mentioned in the manual. (The humidity in the laboratory is 20%-60%, and the temperature is 18°C - 25°C. TMB was balanced to 37°C before color development, and incubated at 37°C for 15 minutes in the dark after adding the enzyme label plate holes.) The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.
Citations
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