Anti-Human IgG2 Fd monoclonal antibody, FITC

Microtiter wells were coated with human immunoglobulins with various isotypes. CABT-L908M was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as secondary antibody.
Result: CABT-L908M does not cross-react with human IgG1, IgG3, IgG4, IgA, IgM and IgE.

IHC-P analysis in human tonsil tissue by anti-human IgG2 antibody.
IHC-P was performed using sections of formalin-fixed paraffin-embedded human tonsil tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with anti-human IgG2 primary antibody at 1 µg/mL at room temperature for 1 h.Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Germinal center cells and squamous epithelial cells are positively stained at cytoplasm.