Product Overview
A DNA sequence encoding the Influenza A virus (A/chicken/VietNam/NCVD-016/2008(H5N1)) hemagglutinin (ACO07033.1) (Met1-Gln532) with cleavage site mutated (RRRK →TET, HA1+HA2,uncleaved) was expressed with a C-terminal polyhistidine tag.
Molecular Weight
The recombinant hemagglutinin of Influenza A virus (A/chicken/VietNam/NCVD-016/2008(H5N1)) comprises 526 amino acids and has a predicted molecular mass of 59.9 kDa. The apparent molecular mass of the protein is approximately 62.1 kDa in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Format
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0.1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Storage
Samples are stable for up to twelve months from date of receipt at -70°CStore it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Keywords
Influenzavirus A; Influenza A virus; Influenza A virus H5N1; H5N1; IAV H5N1
Citations
Publication ()
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COA
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