Recombinant IAV H5N1 (A/Anhui/1/2005) Hemagglutinin (a.a. 18-530) [His]

Product Overview

C-terminal 6xHis tagged HA(H5N1)(A/Anhui/1/2005) protein (a.a.18-530)

Nature

Recombinant

Tag/Conjugate

His

Alternative Names

Influenzavirus A; Influenza A virus; Influenza A virus H5N1; H5N1; IAV H5N1; IAV H5N1 Hemagglutinin; IAV H5N1 HA; H5N1 HA

Purity

≥ 95%

Format

Liquid

Concentration

1 mg/ml

Buffer

PBS

Preservative

None

Storage

After reconstitution, stable for a month at 4?C. For long term storage, split it into small aliquots and keep at -80°C. Avoid repeated freezing and thawing.

Introduction

Hemagglutinin (HA) or Haemagglutinin (BE) is an antigenic glycoprotein found on the surface of the influenza viruses. It is responsible for binding the virus to the cell that is being infected. The name "Hemagglutinin" comes from the protein's ability to cause red blood cells (erythrocytes) to clump together ("agglutinate") in vitro. The process is like this: Hemagglutinin (HA) binds to the monosaccharide sialic acid which is present on the surface of its target host cells. The cell membrane then engulfs the virus through endocytosis and forms endosome. The cell then attempts to begin digesting the contents of the endosome by acidifying its interior and transforming it into a lysosome. When the pH within the endosome drops to 6.0, the HA molecule becomes partially unfold, and releasing a very hydrophobic portion of its peptide chain that was previously hidden within the protein. This so-called "fusion peptide" acts like a molecular grappling hook by inserting itself into the endosomal membrane and locking on. Then, when the rest of the HA molecule refolds into a new structure and pulls the endosomal membrane right up next to the virus particle's own membrane, causing the two to fuse together. Once this has happened, the viral RNA genome enters into the cell's cytoplasm.

Keywords

Influenzavirus A; Influenza A virus; Influenza A virus H5N1; H5N1; IAV H5N1; IAV H5N1 Hemagglutinin; IAV H5N1 HA; H5N1 HA