Influenza A H5N1 (Avian Flu) Hemagglutinin / HA ELISA Pair Set

Species Reactivity

IAV

Detection Method

sELISA

Intended Use

The H5N1 (avian flu) hemagglutinin ELISA Pair Set is for the quantitative determination of H5N1 (avian flu) hemagglutinin. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.

Contents of Kit

Bring all reagents to room temperature before use.
Capture Antibody: 1 mg/mL of mouse anti-H5N1 hemagglutinin monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating.
Detection Antibody: 0.2 mg/mL of rabbit anti-Influenza A H5N1 hemagglutinin polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4°C). Dilute to working concentration of 0.7 μg/mL in dilution buffer before use.
Standard: Each vial contains 299 ng of recombinant H5N1 hemagglutinin. Reconstitute with 1 mL dilution buffer. A seven-point standard curve usi ng 2-fold serial dilutions in dilution buffer, and a high standard of 8000 pg/mL is recommended.

Storage

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4°C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted standard at -80°C for up to 1 month. Avoid repeated freezethaw cycles.

Detection Range

125-8000 pg/mL

Sensitivity

The minimum detectable dose of H5N1 hemagglutinin (HA) was determined to be approximately 125 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

Standard Curve

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.