Product Overview
A DNA sequence encoding the the N-terminal segment (Met 1-Pro 337) of Influenza A virus H5N1 hemagglutinin (A/Anhui/1/2005 (H5N1) (ABD28180.1), termed as HA1, was fused with a polyhistidine tag at the C-terminus.
Molecular Weight
The recombinant HA1 subunit of Influenza A virus H5N1 hemagglutinin (A/Anhui/1/2005 (H5N1) comprises 332 amino acids with the predicted molecular mass of 37.5 kDa. As a result of glycosylation, it migrates as an approximately 55-60 kDa band in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Format
Lyophilized from sterile PBS, pH 7.41. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Storage
Samples are stable for up to twelve months from date of receipt at -70°CStore it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Keywords
Influenzavirus A; Influenza A virus; Influenza A virus H5N1; H5N1; IAV H5N1
Citations
Publication ()
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COA
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