A DNA sequence encoding the Influenza A virus (A/Puerto Rico/8/34/Mount Sinai (H1N1)) nucleoprotein (AAM75159.1) (Met1-Gly490) was fused with a polyhistidine tag at the C-terminus.
Nature
Recombinant
Tag/Conjugate
His
Molecular Weight
The recombinant Influenza A virus (A/Puerto Rico/8/34/Mount Sinai (H1N1)) nucleoprotein comprises 501 amino acids and has a predicted molecular mass of 56.6 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 97 % as determined by SDS-PAGE
Format
Lyophilized from sterile 60mM Tris, 500mM NaCl, pH 7.41. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -70°CStore it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Keywords
Influenzavirus A; Influenza A virus; Influenza A virus H1N1 NP;H1N1 NP; IAV NP
Citations
Publication ()
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