Sample
Serum, plasma, cellculture supernatant and other biological samples.
Intended Use
In vitro quantitative determination of PAPPA concentrations in serum, plasma, cellculture supernatant and other biological samples.
Contents of Kit
| No. | Components | Size | Storage Conditions |
| 1 | ELISA Microplate(Dismountable) | 8×12 | Stored for 1 month at 2-8°C. Stored for 6 month at -20°C. |
| 2 | Lyophilized Standard | 2vial | Stored for 1 month at 2-8°C. Stored for 6 month at -20°C. |
| 3 | Biotin-labeled Antibody(Concentrated, 100×) | 120ul | 2-8°C (Avoid Direct Light) |
| 4 | HRP-Streptavidin Conjugate(SABC, 100×) | 120ul | 2-8°C (Avoid Direct Light) |
| 5 | TMB Substrate | 10ml | 2-8°C (Avoid Direct Light) |
| 6 | Sample Dilution Buffer | 20ml | 2-8°C |
| 7 | Antibody Dilution Buffer | 10ml | 2-8°C |
| 8 | SABC Dilution Buffer | 10ml | 2-8°C |
| 9 | Stop Solution | 10ml | 2-8°C |
| 10 | Wash Buffer(25×) | 30ml | 2-8°C |
Storage
2-8°C (for sealed box), please do not freeze! See kit label for expiry date.
Performance Characteristics
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Detection Range
0.625-40mIU/ml
General Description
Pappalysin-1, also known as pregnancy-associated plasma protein A (PAPP-A), is a zinc metalloproteinase that belongs to the metzincin superfamily of proteases. It plays roles in growth factor signaling, extracellularmatrix remodeling, and tissue development by cleaving insulin-like growth factor-binding proteins (IGFBPs), releasing bioactive insulin-like growth factors (IGFs). Pappalysin-1-mediated proteolysis regulates IGF availability, activity, and receptor signaling, influencing cell proliferation, differentiation, and survival. Dysregulated PAPP-A expression is associated with pregnancy complications, cardiovascular diseases, and cancer progression, highlighting its diagnostic and prognostic value in clinical medicine.
Standard Curve
This product has been tested by Quality Control Department and meets performance specifications mentioned in the manual. (The humidity in the laboratory is 20%-60%, and the temperature is 18°C - 25°C. TMB was balanced to 37°C before color development, and incubated at 37°C for 15 minutes in the darkafter adding the enzyme label plate holes.)
The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.
Citations
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