Human High sensitive Interleukin 12 p70 ELISA Kit

Sample

Serum, plasma, tissue homogenates and other biological fluids

Species Reactivity

Human

Detection Method

sELISA

Intended Use

HS-IL-12 p70 ELISA Kit allows for the in vitro quantitative determination of IL-12 p70 concentrations in serum, plasma, tissue homogenates and other biological fluids.

Contents of Kit

Storage

2-8 °C for 6 months

Precision

Intra-assay:CV<8%;
Inter-assay:CV<10%

Detection Range

7.8-500pg/ml

Sensitivity

< 4.6pg/ml

General Description

IL-12 (interleukin 12) was first described as natural killer stimulating factor in 1989. The heterodimeric cytokine IL-12 consists of a 35-kd light chain (p35 or IL-12A) and 40-kd heavy chain (p40 or IL-12B). The gene encoding p35 is located on chromosome 3 in human beings and on chromosome 6 in mice. The p35 protein contains 197 amino acids and has homology to other single-chain cytokines (eg, IL-6 and G-CSF). The IL-12 p40 gene is on the human chromosome 5 in the same area as IL-3, IL-5, and GM-CSF, and the mice gene is on chromosome 11. P40 has homology to the extracellular domain of members of hematopoietic cytokinereceptor family (eg, IL-6Rα).
IL-12 (interleukin 12) receptor, binding interleukin 12, is composed of two subunits, IL-12RB1 (also known as CD212) and IL12RB2. These IL-12 functions depend on its IL-12 and IL-12 receptor complex. IL-12 (interleukin 12) is a heterodimeric pro-inflammatory cytokine that induces the production of interferon-γ (IFN-γ), favours the differentiation of T helper 1 (TH1) cells and forms a link between innate resistance and adaptive immunity.
Dendritic cells (DCs) and phagocytes produce IL-12 in response to pathogens during infection. Production of IL-12 is dependent on differential mechanisms of regulation of expression of the genes encoding IL-12, patterns of Toll-like receptor (TLR) expression and cross-regulation between the different DC subsets, involving cytokines such as IL-10 and type I IFN.

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