IL-12/ IL-23 p40 ELISA Kit

Sample

serum, plasma, cell culture supernatant

Species Reactivity

Human

Intended Use

The human IL-12/IL-23 p40 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-12/IL-23 p40. The human IL-12/IL-23 p40 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Contents of Kit

Microwell Plate
Biotin-Conjugate: 1 x120μl
Streptavidin-HRP: 1 x150 μl
Standard: 2 x
Sample Diluent: 1 x12 ml
Assay Buffer Concentrate 20x: 1 x5 ml
Wash Buffer Concentrate 20x: 1 x50 ml
Substrate Solution: 1 x15 ml
Stop Solution: 1 x15 ml
Adhesive Films: 4 x

Storage

2-8°C

Precision

Detection Range

31.2 - 2000 pg/mL

Sensitivity

<10 pg/ml

General Description

IL-23 and IL-12 are heterodimeric cytokines which share the p40 subunit, but have unique second subunits, IL-23p19 and IL-12p35. IL-12p70 is a heterodimer composed of a 35-kDa subunit (IL-12p35) and the 40-kDa subunit (IL-12p40) that binds the IL-12 receptor. The p40 subunit is secreted in excess of IL-12p35 and can also associate with p19 (IL-23P19 or IL-23A) to form IL-23. The p19 subunit shares sequence similarity with IL-6 subfamily members and is distantly related to the p35 subunit of IL-12. It shows no biological activity by itself and combines with the p40 subunit of IL-12 to form a biologically active, composite cytokine, IL-23. IL-23 signals through the IL-23 receptor and has functions that overlap with and are distinct from those of IL-12. Unlike IL-12, IL-23 has no effect on naive T cells but selectively activates memory T cells to secrete IFN- and IL-17. IL-23 is more potent than IL-12 in inducing long-lasting T-cell responses to viral antigens and can induce tumor-directed cytotoxic T-cell responses.

Standard Curve