Human Growth Hormone ELISA Kit

Sample

Cell culture supernatants, plasma and serum

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The Human Growth hormone ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of Human Growth hormone in cell culture supernatants, plasma and serum.

Contents of Kit

1. Human Growth hormone Antibody Coated wells, 96-well plate (12 strips of 8 wells), 1 plate
2. Human Growth hormone Biotin Conjugate (80×), 2 vials
3. Human Growth hormone Standard, recombinant Human Growth hormone, 2 vials
4. Wash Buffer Concentrate (20×), 25 mL
5. Assay Diluent A (contains 0.09% sodium azide), 30 mL
6. Assay Diluent B (5×), 15 mL
7. Streptavidin-HRP (200×), 0.2 mL
8. TMB Substrate, 12 mL
9. Stop Solution, 8 mL
10. Adhesive Plate Covers, 2

Storage

Store the kit reagents at -20°C. Expiry of the kit and reagents is stated on the labels. The kit components' expiry is guaranteed only if they are stored properly and not contaminated during repeated use. Do not mix components from different kit lots

Precision

Intra-assay precision
To determine intra-assay precision, two standard curves and 3 samples for each standard curve are run. The standard curve
concentration points as well as the samples are tested in duplicates on a single plate. Two different concentration values
are obtained for each sample, using the two separate standard curves. The two concentration values for each sample is
compared to each other using the CV% calculation. Intra-Assay CV: <10%
Inter-assay precision
To evaluate inter-assay precision, the second standard curve is tested on a separate plate along with the second set of samples. Inter-Assay CV: <12%

Standard Curve

Standard curve (example)
These standard curves are for demonstration only. A standard curve must be run with each assay.