Zebrafish GH (Growth Hormone) ELISA Kit

Sample

Cell culture supernate, serum and plasma

Species Reactivity

Zebrafish

Detection Method

cELISA

Intended Use

This ELISA kit used for quantitation of Zebra Fish Growth Hormone (GH) concentration in serum, plasma and other biological fluids.

Contents of Kit

1. Pre-Coated Microplate 12 strips x 8 wells 4°C/-20°C
2. Standard (Lyophilized) 2 vials 4°C/-20°C
3. Biotinylated Antibody (100×) 120 μL 4°C/-20°C
4. Streptavidin-HRP (100×) 120 μL 4°C/-20°C
5. Standard/Sample Diluent Buffer 20 mL 4°C/-20°C
6. Biotinylated Antibody Diluent 12 mL 4°C/-20°C
7. HRP Diluent 12 mL 4°C/-20°C
8. Wash Buffer (25×) 20 mL 4°C/-20°C
9. TMB Substrate Solution 10 mL 4°C/-20°C (store in dark)
10. Stop Reagent 6 mL 4°C/-20°C
11. Plate Covers 2 pieces RT

Storage

1. Store the Pre-Coated Microplate, Standard ,Biotinylated Antibody and Streptavidin-HRP at -20°C, the rest reagents at 4°C, please used up within 6 months.
*If the kit is not opened, store the whole kit: 4°C(short time storage, valid for 6 months); -20°C (long-term storage, valid for 1 year). Avoid repeated freeze-thaw cycles.
2. Do not use the kit beyond the expiration date.
3. If the whole kit is stored at -20°C, place the kit at 4°C the day before the experiment.

Precision

Intra-assay Precision (Precision within an assay):CV% < 8%
Three samples of known concentration were tested twenty times on 1 plate to assess intra-assay precision.
Inter-assay Precision (precision between assays):CV% < 10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Detection Range

0.16-10 ng/mL

Sensitivity

55.7 pg/mL

General Notes

Hormone Markers ELISA Kits