Human CD5 ELISA Kit

Sample

Cell culture supernates

Species Reactivity

Human

Detection Method

Sandwich-ELISA

Intended Use

The kit is developed for the detection and quantitative determination of Human CD5 in cell culture supernates. It is intended for research use only (RUO). It is designed to provide a reliable solution for cell therapy product quality assessment during drug development and CMC quality control stages.

Contents of Kit

1. Pre-coated Anti-CD5 Antibody Microplate: 1 plate, store at 2-8°C once opened
2. Human CD5 Standard (Lyophilized): 20 μg, store at -70°C once opened
3. Biotin-Anti-CD5 Antibody (Lyophilized): 15 μg, store at -70°C once opened
4. Streptavidin-HRP (Lyophilized): 10 μg, store at -70°C (avoid light) once opened
5. 20× Washing Buffer: 50 mL, store at 2-8°C once opened
6. 1× Dilution Buffer: 50 mL, store at 2-8°C once opened
7. Substrate Solution: 12 mL, store at 2-8°C (avoid light) once opened
8. Stop Solution: 7 mL, store at 2-8°C once opened

Storage

1. Unopened kit should be stored at 2°C-8°C upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per Reagents And Materials Provided. The shelf life is 30 days from the date of opening.

Precision

Intra-assay Precision: Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.
Inter-assay Precision: Three samples of known concentration were tested in three separate assays to assess inter-assay precision.

Sensitivity

The minimum detectable concentration of CD5 is 6.51 pg/mL. The minimum detectable concentration was determined by adding twice standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Standard Curve