Intended Use
Anti-SSA IgG ELISA provides a rapid quantitative measurement of Anti-SSA IgG in serum to further investigate the presence of specific autoantibodies. The assay is intended for in vitro research use only (RUO).
Contents of Kit
1. Nuclear antigen coated microwell strips (96 wells),
2. Anti-SSA IgG Stds. A-F in a serum buffer (0, 12.5, 25, 50, 100, and 200 U/ml), 6 vials x 1.5 ml each
3. Anti-SSA IgG Negative Control (1.5 ml each)
4. Anti-SSA IgG Positive Control (1.5 ml each)
5. Sample Diluent (5X), 20 ml (yellow color)
6. Wash buffer (50X), 20 ml
7. Anti-hIgG HRP Conjugate, 15 ml (light red)
8. HRP Substrate Solution (TMB) , 15 ml
9. Stop Solution, 15 ml
10. Complete Instruction Manual
Storage
The microtiter well plate and all other reagents are stable at 2-8°C until the expiration date printed on the label. The whole kit stability is usually 12 months from the date of shipping under appropriate storage conditions.
Do not contaminate the bottles. Withdraw solutions in a separate clean tube or dispensing trays. Any unused solution should be discarded and not returned to the bottle. Do not use HRP substrate solution if this solution is blue. Do not expose these solutions to strong light.
Sensitivity
The lower detection limit for Anti-SS-A has been determined at 1.0 U/ml.
General Description
Rheumatoid autoimmune diseases are often associated with the occurrence of autoantibodies against several nuclear or cytoplasmatic antigens. In patients with Sjögren´s syndrome antibodies against SS-A and SS-B often occur in combination. Anti SS-A antibodies pass the placenta and may cause the development of SLE in neonates. Each class of immunoglobulins causes a specific immunofluorescence pattern. Basically immunofluorescence titers correlate with the quantitation of IgG antibodies but the concentrations may vary considerably within each titer. Quantitation of IgG class antibodies extensively correlates with the disease activity. This makes quantitative ELISA tests superior to immunofluorescence using Hep2 cells.
Today the best investigated immunoreactive antigens are double-stranded DNA (dsDNA), single stranded DNA (ssDNA), Sm (Smith), sn-RNP (small nuclear ribonucleoprotein particles), the complex RNP/Sm which is stabilized by ribonucleic acid as well as SS-A (Ro) and SS-B (La). The antigen Scl 70, a 70 kD molecular weight protein is associated with scleroderma. In rheumatoid autoimmune diseases various profiles of autoantibodies to these antigens can be detected. They are related with high incidence to active and inactive systemic lupus erythematosus, mixed connective tissue diseases (Sharp syndrome), rheumatoid arthritis, Sjögren´s syndrome, scleroderma, photosensitive dermatitis and druginduced lupus. In lupus patients typically anti-dsDNA antibodies can be detected. Patients without these antibodies very often show anti-ssDNA antibodies and anti-SS-A and anti SS-B are present. A strong correlation between antibody concentration and severity of the disease has been observed with higher antibody concentrations in active phases of the disease. Thus quantitation is more informative compared to simple titering by immunofluorescence. Most of these parameters are not specific for a single disease but they occur in various combinations. The pattern of different antibody combinations and their concentration together with the whole clinical picture of the patient are helpful diagnostic tools in the assessment of rheumatoid autoimmune diseases.
Citations
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