Specifications
Species Reactivity
Human, Rat
Immunogen
A synthetic peptide of human GRP78 BiP
Applications
Application Notes
WB: 1/500-1/1000 IHC: 1/50-1/100
Each laboratory should determine an optimum working titer for use in its particular application.
Other applications have not been tested but use in such assays should not necessarily be excluded.
Images
Western blot analysis of GRP78 BiP in C6 lysates using GRP78 BiP antibody. (Cat.No DMAB-JX2371004)
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using GRP78 BiP antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. (Cat.No DMAB-JX2371004)
Target
Alternative Names
HSPA5; GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.
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Citations
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Fae, KC; da Silva, DD; et al. PDIA3, HSPA5 and vimentin, proteins identified by 2-DE in the valvular tissue, are the target antigens of peripheral and heart infiltrating T cells from chronic rheumatic heart disease patients. JOURNAL OF AUTOIMMUNITY 31:136-141(2008).
Mande, PV; Thomas, S; et al. Immunization with ovarian autoantigens leads to reduced fertility in mice following follicular dysfunction. REPRODUCTION 143:309-323(2012).