Human HSPA5/GRP78/BiP ELISA Kit (DEIABL15)

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Size
96T
Sample
cell culture supernatants, cell Lysates, plasma, serum, tissue homogenates
Species Reactivity
Human
Intended Use
The Human HSPA5/GRP78/BiP ELISA Kit is a sandwich ELISA for the quantitative measurement of human HSPA5/GRP78/BiP in cell culture supernatants, cell Lysates, plasma, serum and tissue homogenates.
Contents of Kit
1. Coated 96-well Strip Plate
2. Adhesive Plate Sealers
3. Standard (Lyophilized)
4. Sample Diluent
5. Detection Reagent A (Green)
6. Detection Reagent B (Red)
7. Assay Diluent A
8. Assay Diluent B
9. TMB Substrate
10. Stop Solution
11. Wash Buffer (30 × concentrate)
12. Quality Control (Lyophilized) Expected Value: 2.49 ng/ml
Storage
4°C
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock 70kDa Protein 5 (HSPA5) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock 70kDa Protein 5 (HSPA5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Detection Range
0.313-20ng/Ml
Sensitivity
0.119ng/mL
Standard Curve

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