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VHH is the variable domain of camelids heavy chain antibodies, and it is also called single-domain antibody (sdAb) or nanobody. Due to the large size, traditional secondary antibodies hinder tissue penetration and reduce image resolution in super-resolution fluorescence microscopy which made VHHs/SdAbs an ideal candidate to replace such reagents. Additionally, site-specifically labeled SdAbs can place fluorophores closer than 2 nm to the antigen or even tolerate up to three dyes. Combining excellent antigen specificity, minimal lot-to-lot variation, and sustainable supplement, VHHs/SdAbs is considered to be a promising tool for super-resolution imaging.
Fig. 1 Schematic diagram for the primary structure of a conventional antibody, heavy-chain antibody (HCAb), nanobody, and small, recombinant antibody.
Creative Diagnostics offers HiResNb™ secondary VHH antibodies with multiple conjugations, which have excellent stability, specificity, and sensitivity, and have been verified in a variety of applications.
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Fig. 2 Specificity for different species of IgG (DMABB-JP10)
Fig. 3 A titer ELISA of mouse IgM. (DMABB-JP73)
Fig. 4 A titer ELISA of mouse IgG2a. (DMABB-JP51)
Fig. 5 A titer ELISA of mouse IgG2b. (DMABB-JP57)
Fig. 6 Indirect immunostaining of PFA fixed mouse hippocampus neurons. (DMABB-JP99)
Fig. 7 Indirect immunostaining of PFA fixed Cos7 cells. (DMABB-JP100)
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Fig. 8 Specificity for different species of IgG (DMABB-JP80)
Fig. 9 A titer ELISA of Rabbit IgG. (DMABB-JP80)
Fig. 10 Indirect immunostaining of PFA fixed Cos7 cells. (DMABB-JP101)
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Fig. 11 Specificity for different species of IgG (DMABB-JP10)
Fig. 12 A titer ELISA of Human IgG. (DMABB-JP10)
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Fig. 13 Specificity for different species of IgG (DMABB-JP01)
Fig. 14 A titer ELISA of chicken IgY. (DMABB-JP01)
Fig. 15 Indirect immunostaining of PFA fixed Hela cells. (DMABB-JP96)
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| HiResNb™ Anti-Chicken IgY Secondary Antibodies | |||
| Cat. No. | Product Name | Application | |
| DMABB-JP01 | HiResNb™ Anti-Chicken IgY VHH antibody, clone 135-212 | WB, ELISA | Inquiry |
| DMABB-JP03 | HiResNb™ Anti-Chicken IgY VHH antibody, clone 135-212 [HRP] | WB, ELISA | Inquiry |
| DMABB-JP02 | HiResNb™ Anti-Chicken IgY VHH antibody, clone 135-212 [Biotin] | ELISA | Inquiry |
| DMABB-JP96 | HiResNb™ Anti-Chicken IgY VHH antibody, clone 4H3 [Alexa Fluor 568] | IF, WB | Inquiry |
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Fig. 16 Specificity for different species of IgG (DMABB-JP04)
Fig. 17 A titer ELISA of Goat IgG. (DMABB-JP04)
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| HiResNb™ Anti-Goat IgG Secondary Antibodies | |||
| Cat. No. | Product Name | Application | |
| DMABB-JP07 | HiResNb™ Anti-Goat IgG(H+L) VHH antibody, clone 165-213 | WB, ELISA | Inquiry |
| DMABB-JP09 | HiResNb™ Anti-Goat IgG(H+L) VHH antibody, clone 165-213 [HRP] | WB, ELISA | Inquiry |
| DMABB-JP08 | HiResNb™ Anti-Goat IgG(H+L) VHH antibody, clone 165-213 [Biotin] | WB, ELISA, IP | Inquiry |
| DMABB-JP04 | HiResNb™ Anti-Goat IgG(Fcγ specific) VHH antibody, clone 165-212 | WB, ELISA | Inquiry |
| DMABB-JP06 | HiResNb™ Anti-Goat IgG(Fcγ specific) VHH antibody, clone 165-212 [HRP] | WB, ELISA | Inquiry |
| DMABB-JP05 | HiResNb™ Anti-Goat IgG(Fcγ specific) VHH antibody, clone 165-212 [Biotin] | WB, ELISA | Inquiry |
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