Specifications
Immunogen
Recombinant Fc region of mouse IgG2b
Applications
Application Notes
ELISA: 1:10000-1:50000 WB: 1:10000-1:50000 IP: 1-2ug/sample
Each laboratory should determine an optimum working titer for use in its particular application.
Other applications have not been tested but use in such assays should not necessarily be excluded.
Images
A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. 1:10000 dilution of Anti-Mouse IgG2b(Fcγ Fragment specific) VHH (Biotin) was used as the primary antibody. An HRP conjugated streptavidin as the secondary antibody.
ELISA of specificity for different species of IgG. The plate was coated with 2ug/ml of different IgG. 1:1000 dilution of Anti-Mouse IgG2b(Fcγ Fragment specific) VHH(Biotin) was used as the primary antibody. An HRP conjugated streptavidin as the secondary antibody.
Target
Alternative Names
IGHG2B; immunoglobulin heavy constant gamma 2B; IgG2b; Igh-3; gamma2b;
Citations
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Acharya, P; Luongo, TS; et al. Heavy Chain-Only IgG2b Llama Antibody Effects Near-Pan HIV-1 Neutralization by Recognizing a CD4-Induced Epitope That Includes Elements of Coreceptor- and CD4-Binding Sites. JOURNAL OF VIROLOGY 87:10173-10181(2013).
Yang, ZG; Sun, HX; et al. Haemolytic activities and adjuvant effect of Astragalus membranaceus saponins (AMS) on the immune responses to ovalbumin in mice. VACCINE 23:5196-5203(2005).