High Sensitivity Rat 8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit

Name: High Sensitivity Rat 8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit
SKU: DEIA-BYF2638
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, plasma, cell culture supernatant and other biological samples

Species Reactivity

Rat

Detection Method

Competitive-ELISA

Intended Use

In vitro quantitative determination of 8-OHdG concentrations in serum, plasma, urine, cell culture supernatant and other biological samples.

Contents of Kit

No.	Components	Size	Storage Conditions
1	ELISA Microplate(Dismountable)	96T (8×12)	2-8°C/-20°C
2	Lyophilized Standard	2 vials	2-8°C/-20°C
3	Biotin-labeled Antibody (Lyophilized)	1 vial	2-8°C/-20°C
4	HRP-labeled Conjugate (SABC, 100X)	120ul	2-8°C (Avoid Direct Light)
5	TMB Substrate	10ml	2-8°C (Avoid Direct Light)
6	Purified water	200ul	2-8°C
7	Sample Dilution Buffer	20ml	2-8°C
8	Antibody Dilution Buffer	10ml	2-8°C
9	SABC Dilution Buffer	10ml	2-8°C
10	Stop Solution	10ml	2-8°C
11	Wash Buffer(25X)	30ml	2-8°C
12	Plate Sealer	5 pieces

Storage

2-8°C (for sealed box), please do not freeze! See kit label for expiry date.

Performance Characteristics

Stability: Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Detection Range

0.468-30ng/ml

Sensitivity

0.281ng/ml

General Description

CAS : 88847-89-6
8-Hydroxydeoxyguanosine is a nucleoside modification that arises from DNA oxidation and is one of the markers of DNA oxidative damage. DNA oxidative damage is a common type of DNA damage that is closely associated with many diseases, such as cancer, neurodegenerative diseases, and others. The level of 8 hydroxydeoxyguanosine can be detected in vivo or ex vivo to assess the degree of DNA oxidation and oxidative stress status, and it can also be used as a potential biomarker. In biochemistry, 8 hydroxydeoxyguanosine is also used as an important indicator in DNA damage repair studies, as it can cause damage types requiring repair, such as mismatched base pairing and DNA strand breakage.