Anti-8-OHdG monoclonal antibody (DMAB15633)

Specifications


Host Species
Mouse
Antibody Isotype
IgG2a
Clone
26B4
Species Reactivity
N/A
Immunogen
8-hydroxy-guanosine conjugated with BSA and casein.
Conjugate
Unconjugated

Applications


Application Notes
Immunohistochemistry (1:200-1:1000) & The optimal working dilution should be determined by the end user.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
8-Oxo-2"-deoxyguanosine; 8-oxo-dG; Mouse Anti-8-hydroxy-guanosine Monoclonal Antibody; Anti-8-hydroxy-guanosine Monoclonal Antibody; 8-hydroxy-guanosine Monoclonal Antibody Mouse Anti-8-hydroxy-guanosine MAb; Anti-8-hydroxy-guanosine MAb

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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


NanoTiO(2) Sunscreen Does Not Prevent Systemic Oxidative Stress Caused by UV Radiation and a Minor Amount of NanoTiO(2) is Absorbed in Humans

NANOMATERIALS

Authors: Pelclova, Daniela; Navratil, Tomas; Kacerova, Tereza; Zamostna, Blanka; Fenclova, Zdenka; Vlckova, Stepanka; Kacer, Petr

The present pilot study tested the efficiency of nanoTiO(2) sunscreen to prevent the oxidative stress/inflammation caused by ultraviolet (UV) radiation using biomarkers in subjects' blood, urine, and exhaled breath condensate (EBC). In addition, the skin absorption of nanoTiO(2) was studied. Six identical subjects participated in three tests: (A) nanoTiO(2) sunscreen, (B) UV radiation, and (C) sunscreen + UV. The first samples were collected before the test and the second after sunscreen application and/or UV exposure. On day 4, the third samples were collected, and the sunscreen was washed off, and the fourth samples were collected on day 11. The following biomarkers were measured: malondialdehyde, 4-hydroxy-trans-hexenal, 4-hydroxy-trans-nonenal, aldehydes C6-C12, 8-iso-Prostaglandin F2 alpha, o-tyrosine, 3-chlorotyrosine, 3-nitrotyrosine, 8-hydroxy-2-deoxyguanosine, 8-hydroxyguanosine, 5-hydroxymethyl uracil, and leukotrienes, using liquid chromatography-electrospray ionisation-tandem mass spectrometry. Titania was measured using inductively coupled plasma mass spectrometry and TiO2 nanoparticles by transmission and scanning electron microscopy. Sunscreen alone did not elevate the markers, but UV increased the biomarkers in the plasma, urine, and EBC. The sunscreen prevented skin redness, however it did not inhibit the elevation of oxidative stress/inflammatory markers. Titania and nanoTiO(2) particles were found in the plasma and urine (but not in the EBC) in all sunscreen users, suggesting their skin absorption.

Recognition and detection of 8-oxo-rG in RNA using the DNA/OMeRNA chimera probes containing fluorescent adenosine-diazaphenoxazine analog

BIOORGANIC & MEDICINAL CHEMISTRY

Authors: Koga, Yohei; Taniguchi, Yosuke; Kikukawa, Yoshiya; Sasaki, Shigeki

Recent studies indicate that oxidative damage to RNA results in dysfunction of translation and eventual pathogenesis. A representative oxidized base in RNA is 8-hydroxyguanosine (8-oxo-rG), however, unlike its DNA counterpart (8-oxo-dG), its role in pathogenesis has not attracted much attention until recently. The 2'-deoxyadenosine derivative with a diazaphenoxazine skeleton at the 6 -amino group (Adap) was shown to be selective for 8-oxo-dG in DNA. In this study, the 2'-0-methoxy derivative of Adap (2'-0MeAdap) was designed as a selective molecule for 8-oxo-rG in RNA. 8-Oxo-rG in the homopurine RNA was selectively recognized by the ODN probe incorporating Adap. In contrast, although it was not possible by the Adap-containing ODN prove due to the instability of the corresponding duplex, 8-oxorG in homopyrimidine RNA was selectively detected by the 2'-0MeRNA probe incorporating 2'-0MeAdap. (C) 2016 Published by Elsevier Ltd.

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