RHA™ anti-Fenpropathrin monoclonal antibody, clone FPP

A method was developed using dispersive solid-phase extraction based on hyperbranched polymer functionalized with beta-cyclodextrin for extraction and enrichment of three pyrethroid pesticides (fenpropathrin, cyhalothrin, and ethofenprox) in environmental water samples before high-performance liquid chromatography analysis. The aim of this research was to establish a simple, high-speed, effective, and sensitive method for the detection of target analytes. The as-prepared sorbents were synthesized successfully, and characterized by scanning electron microscopy, energy dispersive spectroscopy, elemental analysis, Brunauer-Emmett-Teller measurement and Fourier transform infrared spectroscopy. The experimental factors influencing the extraction efficiency such as extraction time, pH, ionic strength, desorption conditions, etc. were optimized. Good linearity was observed for all three pyrethroids, and the linear range of fenpropathrin was 10-500 ng/mL, and cyhalothrin, ethofenprox were 5-500 ng/mL. The method detection limits were ranged from 1.0 ng/mL to 2.1 ng/mL under the optimal conditions. The recoveries achieved ranged from 83.1% to 91.6% for the extraction of the three target analytes. Apart from these, the relative standard deviation (intra-day and inter-day) were measured fewer than 6.0%. The method in this work was applied to the rapid enrichment and detection of pyrethroids in environmental water samples.

The Asian citrus psyllid, Diophorina citri Kuwayama (Hemiptera: Liviidae) threatens California's citrus industry because of its ability to transmit the bacterium, Candidates Liberibacter asiaticus, associated with huanglongbing (HLB). To reduce the risk of HLB spread, an area-wide management strategy was established to control D. citri in both conventional and organic commercial citrus. In addition, releases of the parasitoid Tamarixia radiata Waterston (Hymenoptera: Eulophidae) as a classical biological control agent have been conducted in certain urban areas of California to improve control of D. cirri. The objectives of this study were to document the residual efficacy of organic insecticides against D. citri nymphs, compare the efficacy of multiple applications of the organic spinosad relative to a single application of the non-organic insecticide fenpropathrin, and estimate the non-target effect of selected foliar insecticides on T. radiate. Spinosad + oil showed greater residual control of D. citri nymphs compared to pyrethrins + oil. The effects of organic insecticides were short lived; by 10 d post treatment there was no detectable effect. Fenpropathrin provided significantly longer control of D. citri nymphs and the efficacy of two or more applications of spinosad was comparable to one application of fenpropathrin. Of the organic insecticides, spinosad + oil resulted in the greatest non-target effects on T. radiate and pyrethrins, pyrethrins + oil, and oil alone had the least severe effects on T. radiate. This study provides important information regarding the residual control of D. cirri by organic insecticides and their compatibility with the biological control agent T. radiate.