Fenpropathrin [BSA]

Excessive use of herbicide and insecticide causes bioaccumulation in the environment and increases potential toxicity for people and animals. Portable systems for rapid assays of herbicide and insecticide residues have attracted prominent interests. Here, we developed a two-dimensional (2D) Pt-Ni(OH)(2) nanosheets (NSs) amplified two-way lateral flow immunoassay (LFI) with a smartphone-based readout for simultaneous detection of acetochlor and fenpropathrin. The 2D Pt-Ni(OH)(2) NSs were synthesized and used as the enhanced signal label in the immunoassay due to their high peroxidase-like activity and low migration speed. The two-way LFI was designed to eliminate potential cross-reaction between two targets. Portable detection system was developed based on a smartphone-based readout, which scans the LFI and provides the accurate testing result. The universal use of smartphones makes the developed platform suitable for cheap and on-site applications. Using the integrated platform, detection of acetochlor and fenpropathrin simultaneously was successfully achieved with the detection limits of 0.63 ng/mL and 0.24 ng/mL, respectively. To confirm the performance of the on-site application, we detected 10 non-spiked samples and 3 spiked samples. The obtained detection results were consistent with the data from gas chromatography analysis. The estimated recoveries ranged from 97.12% to 111.46%, indicating the practical reliability of our developed assay. The developed smartphone-based platform exhibits enhanced sensitivity, which provides a promising technique for on-site, multiplex, highly sensitive detection of pesticides.

Cytochrome P450 monooxygenases (P450s), as an important family of detoxification enzymes, participate in the metabolism of agrochemicals in almost all agricultural pests and play important roles in the development of insecticide resistance. Two P450 genes (CYP389B1 and CYP392A26) were identified and their expression patterns were investigated in our previous study. In this study, four more P450 gene sequences (CYP391A1, CYP384A1, CYP392D11 and CYP392A28) from the Clan 2, Clan 3 and Clan 4 families were identified and characterized. Quantitative PCR analysis showed that these four P450 genes were highly expressed in a fenpropathrin-resistant (FeR) strain of Tetranychus cinnabarinus. In addition, their expressions were much more sensitive to fenpropathrin induction in the FeR strain than the susceptible strain. Gene-silencing experiments via double-stranded RNA feeding were carried out. The results showed that mRNA levels of these six P450 genes were reduced in the FeR strain and the activities of P450s were decreased. Consequently mite susceptibilities to fenpropathrin were increased. Interestingly, silencing all six P450 genes simultaneously had an even greater effect on resistance than silencing them individually. This study increases our understanding of the molecular mechanisms of insecticide detoxification, suggesting that the overexpression of these six P450 genes might play important roles in fenpropathrin resistance in T. cinnabarinus collaboratively.