Dinitropheno (Rat) IgG ELISA Kit

Name: Dinitropheno (Rat) IgG ELISA Kit
SKU: DEIASL083
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, plasma

Species Reactivity

Rat

Detection Method

Indirect-ELISA

Intended Use

This test kit is intended for measurement of rat anti-DNP IgG in serum and plasma samples.

Contents of Kit

No.	Components	Size	Storage Conditions
1	DNP-BSA Coated 96-well Plate	12 strips of 8 wells	2-8°C
2	Anti-Rat IgG HRP Conjugate Stock	11 mL	2-8°C
3	Reference Standard (lyophilized)	1 vial	2-8°C
4	20× Wash Solution	50 mL	2-8°C
5	Diluent	50 mL	2-8°C
6	TMB Reagent	11 mL	2-8°C
7	Stop Solution (1N HCl)	11 ml	2-8°C

Storage

The kit should be stored at 2-8°C. The microtiter plate should be kept in a sealed bag with desiccant to minimize exposure to damp air. Test kits will remain stable for six months from the date of purchase provided that the components are stored as described above.

General Description

Haptens such as dinitrophenol (DNP), when attached to carrier proteins such as ovalbumin or keyhole limpet hemocyanin (KLH) and injected into animals, produce a strong immune response. By measuring changes in the levels of anti-DNP IgM and IgG in appropriate animal models, researchers can assess the impact of pharmacologic or genetic manipulations on the immune system. Creative Diagnostics, Inc. offers a simple ELISA for measurement of rat anti-DNP IgG. We also manufacture ELISA kits for rat anti-DNP IgM and mouse anti-DNP IgM and IgG.

Standard Curve

A typical standard curve with optical density readings at 450nm on the Y-axis against anti-DNP IgG concentrations on the X-axis is shown below. This curve is for the purpose of illustration only and should not be used to calculate unknowns.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

Immunoassays for Process-related Impurities

QA & Reviews

Customer Reviews

References

Gangliosides GM1 and GD1a normalize respiratory rates of rat brain mitochondria reduced by tert-butyl hydroperoxide

JOURNAL OF EVOLUTIONARY BIOCHEMISTRY AND PHYSIOLOGY

Authors: Korotkov, S. M.; Sokolova, T. V.; Avrova, N. F.

Abstract

The rate of oxygen consumption by glutamate-and malate-energized rat brain mitochondria, which was stimulated by an uncoupler 2,4-dinitrophenol (DNP), declined in the presence of a prooxidant tert-butyl hydroperoxide. Preincubation with gangliosides GM1 or GD1a at micromolar (but not nanomolar) concentrations significantly slowed down this decline in the mitochondrial respiration, as shown by measuring absolute respiratory rates and ratios of the mitochondrial respiratory rate in the presence of DNP to the basal respiratory rate (V (DNP)/V-0). Gangliosides GM1 and GD1a also slowed down a decline in the DNP-stimulated mitochondrial respiration induced by long-term incubation ("aging") of mitochondria on ice. The data obtained are likely to reflect a prooxidant-induced reduction in the activity of enzymes of the mitochondrial respiratory chain as well as a GM1-and GD1a-induced decrease in the degree of their inactivation. Interestingly, in the presence of the Trk receptor tyrosine kinase inhibitor (K252a) this effect of gangliosides was not manifested in any way. Our data suggest that the direct impact of gangliosides on mitochondrial signaling pathways, specifically on the Trk receptor tyrosine kinase, plays a certain role in the mechanism of their protective effect on cerebral neurons and, probably, neuroglia.

DNP and ATP induced alteration in disease development of Phomopsis longanae Chi-inoculated longan fruit by acting on energy status and reactive oxygen species production-scavenging system

FOOD CHEMISTRY

Authors: Lin, Yifen; Chen, Mengyin; Lin, Hetong; Hung, Yen-Con; Lin, Yixiong; Chen, Yihui; Wang, Hui; Shi, John

Abstract

As compared with P. longanae-inoculated longans, DNP treatment for P. longanae-inoculated longans exhibited higher fruit disease index and pericarp browning index, lower ATP amount and energy charge level, lower activities of SOD, CAT and APX, lower amounts of AsA and GSH, lower levels of DPPH radical scavenging activity and reducing power, higher O-2(-center dot) generating rate and MDA amount. However, supply of ATP for P. longanae-inoculated longans showed the contrary effects. These results gave convincing evidence that DNP treatment for accelerating pericarp browning and disease development of harvested longans caused by P. longanae was due to decreases of energy production and ROS scavenging capacity, and increases of O-2(-center dot) accumulation and membrane lipid peroxidation. Whereas, supply of ATP for retarding pericarp browning and disease development of harvested longans caused by P. longanae was due to increases of energy production and ROS scavenging capacity, and reductions of O-2(-center dot) accumulation and membrane lipid peroxidation. (C) 2017 Elsevier Ltd. All rights reserved.