Diclazuril standard

The effect of subcutaneously injected diclazuril on the output of Eimeria species oocysts was studied in experimentally infected rabbits. Diclazuril was administered either prophylactically at 0.5, 1 or 2 mg/kg bodyweight two days before each rabbit was inoculated with 20,000 oocysts of a mixed-species field isolate of Eimeria or therapeutically at 1, 2 or 4 mg/kg bodyweight five days after they were inoculated. The prophylactic treatments significantly reduced (P < 0.05) the output of oocysts in faeces and the numbers in the rabbits' livers at all doses. The therapeutic treatment at 4 mg/kg also caused a significant reduction (P < 0.05) in oocyst shedding, but the lower doses resulted in only a moderate reduction. The shedding of the pathogenic species Eimeria stiedae, Eimeria magna, Eimeria irresidua, Eimeria flavescens, Eimeria piriformis and Eimeria intestinalis was significantly reduced (P < 0.05) in all the treated groups. The burden of oocysts in the livers of the therapeutic groups (4000 to 9000) were significantly lower (P < 0.05) than in the inoculated but untreated control group (23,000), but higher than in the prophylactic groups (around 1000).

To investigate the anticoccidial effect of diclazuril (Clinacox premix, Janssen Pharmaceut.), day-old turkey poults (9507 cocks and 9315 layers) hatched from eggs of a Mycoplasma free parent flock were divided into two groups. One of the groups received diclazuril (1 mg effective substance/kg of feed, i.e. 200 g Clinacox premix/ton of feed) while the other received lasalocid-Na (100 mg effective substance/kg of feed) up to the age of 84 days. The losses observed in both groups did not exceed the usual grade (5.10 to 5.85 % in case of cocks and 2.70 to 2.78% in case of layers), neither pathological changes of coccidiosis were observed. Body-mass at slaughtering of 117 and 121 days old layers, as well as 139 and 153 days old cocks did not or hardly differed each other in the groups fed with the two anticoccidial drugs (Table). After having received these favorable results, the control of coccidiosis of 453 thousand day-old turkey poults was carried out with diclazuril in the following year.