Diclazuril ELISA Kit

Name: Diclazuril ELISA Kit
SKU: DEIABL-QB39
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Tissue (pork, chicken)

Detection Method

cELISA

Intended Use

This kit can be used in quantitative and qualitative analysis of Diclazuril residue in tissue (pork, chicken).

Contents of Kit

1.Microtiter plate coated with antigen, 96wells
2.Standard solutions (5×1ml/bottle)
0ppb,1ppb,3ppb,9ppb,27ppb
3.Spiking standard solution: (1ml/bottle) ………10ppm
4.Enzyme conjugate 7ml……………….….red cap
5.Antibody solution 7ml...........................green cap
6.Solution A 7ml………………..….…………white cap
7.Solution B 7ml…………………..…….…...…red cap
8.Stop solution 7ml………………….………yellow cap
9.20× Concentrated wash solution 40ml……….…transparent cap
10.2× Concentrated extraction solution 50ml......blue cap

Storage

Store at 2-8°C for 12 months.

Precision

Variation coefficient of the ELISA kit is less than 10%.
Accuracy:
Tissue............................……………………………95±15%

Detection Limit

Tissue (High LOD) ………………………………………8ppb
Tissue (Low LOD)…………………………………...…32ppb

Sensitivity

Test Sensitivity: 1 ppb

General Description

Diclazuril belongs to pyridines compounds. Because the Diclazuril has the broad-spectrum insect resistance, it becomes the most widely used coccidiostat. However, the irrational or intensive usage of diclazuril may result in residues in animal products, consequently not only cause potential hazards to human health but also increase drug resistance of coccidian.
This kit is a new product based on ELISA. It needs 45min in one operation. It can considerably minimize operation error and work intensity.

Standard Curve

To draw a standard curve: take the absorbance value of standards as y-axis, semi logarithmic of the concentration of the Diclazuril standards solution (ppb) as x-axis.
The Diclazuril concentration of each sample (ppb), which can be read from the calibration curve, is multiplied by the corresponding Dilution factor of each sample followed, and the actual concentration of sample is obtained.
Please notice:
For data analysis of the ELISA kits, special software has been developed, which can be ordered on request.
Diluents ratio:
Tissue (High LOD)…………………………...........…8
Tissue (Low LOD)…………………………..………32

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Development of a Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay for the Analysis of Diclazuril in Chicken Tissues

FOOD ANALYTICAL METHODS

Authors: Wang, Yahui; Wang, Zhanhui; Jiang, Haiyang; Xia, Xi; Shen, Jianzhong; Ding, Shuangyang

Abstract

A highly sensitive and specific monoclonal antibody (Mab) against diclazuril was produced. The hapten with diclazuril coupled to diazotised 4-aminobenzoic acid was synthesized and conjugated to bovine serum albumin by the active ester method to form an immunogen for antibody generation. A novel diclazuril carboxymethyloxime derivative used in an ovalbumin conjugate was applied as a heterologous coating antigen and was expected to improve the immunoassay sensitivity. A sensitive and simple indirect competitive enzyme-linked immunosorbent assay (icELISA) based on the Mab for the determination of diclazuril was developed. Under the optimized conditions, the icELISA for diclazuril showed a half maximum inhibition concentration (IC50) value of 1.8 ng/mL, with limit of detection of 0.24 ng/mL and negligible cross-reactivities with other coccidiostat compounds including toltrazuril, robenidine, nicarbazin, halofuginone, amprolium, monensin, and maduramycin. The icELISA was successfully applied to diclazuril residue analysis in spiked chicken tissues. The average recoveries, intra-assay, and inter-assay coefficients of variation were in a range from 77.6 to 103.7 %, 3.7 to 13.0 %, and 5.6 to 18.3 %, respectively.

Simple determination of residual anticoccidial drugs (diclazuril and nicarbazin) in chicken tissues by HPLC

JOURNAL OF THE FOOD HYGIENIC SOCIETY OF JAPAN

Authors: Kanda, M; Ushiyama, K; Igusa, K; Murayama, M; Horie, M; Hirokado, M; Miyazaki, T

Abstract

A simple and rapid determination of anticoccidial drug residues, diclazuril (DCZ) and nicarbazin (NCZ), in chicken tissues has been developed. DCZ and NCZ were extracted with acetonitrile from chicken liver, muscle, and fat. The extract was rinsed with n-hexane saturated with acetonitrile and then evaporated. The residue was dissolved in 1.4 mL of acetonitrile-methanol (1 : 1), then 1.0 mL of n-hexane saturated with acetonitrile-methanol (1 : 1) was added, and the mixture was partitioned by the addition of 0.6 mL of water. DCZ and NCZ in the aqueous layers were determined by HPLC on an Xterra RP-18 column with acetonitrile-0.5% ammonium acetate containing 0.01 mol/L tetra-n-butylammonium hydrogen sulfate (43: 57) as the mobile phase. The mean recoveries (n=5) of DCZ and NCZ spiked in chicken tissues at the maximum residue levels were 92.0similar to95.6% (CV 2.4similar to3.0%) and 87.3similar to89.4% (CV 1.7similar to2.8%), respectively. The detection limits of DCZ and NCZ were 0.01 and 0.004 mug/g, respectively.