Cocaine/BZE Oral Fluid ELISA Kit

Sample

human oral fluid

Species Reactivity

Human; Research Animal

Detection Method

cELISA

Intended Use

For the determination of trace quantities of Cocaine, Benzoylecgonine (BZE) and/or other metabolites in human oral fluid.

Contents of Kit

1. Wash Buffer Concentrate (10X): 20 mL. Phosphate buffered saline solution with a surfactant. Dilute 10 fold with deionized or ultrapure water before use. Diluted wash buffer is used to wash all unbound conjugate and samples from the plate after the conjugate incubation.
2. K-Blue Substrate: 20 mL (ready-to-use). Stabilized 3,3', 5,5'Tetramethylbenzidine (TMB) plus Hydrogen Peroxide (H₂O₂) in a single bottle. It is used to develop the color in the wells after washing. Light Sensitive.
3. Drug-Enzyme Conjugate: 14 mL (ready-to-use). Drug-horseradish peroxidase concentrate. Do not dilute.
4. Antibody Coated Plate: A 96 well Costar plate, in strips of 8 break-away wells, coated with anti-drug antiserum. The plate is ready for use as is. Do not wash.
5. Acid Stop Solution: 14 mL (ready-to-use). 1 N H₂SO₄ used to stop the enzyme reaction.

Storage

This kit can be used until the expiration date on the label when stored refrigerated at 2-8°C

Sensitivity

The term I-50 is used to define the sensitivity of the test. This number is derived from a standard curve generated with the drug. The drug concentration that shows 50% less color activity than the zero standard is considered to be the I-50.

General Description

The Cocaine/BZE Oral Fluid ELISA Kit is a qualitative one-step kit designed for use as a screening device for the detection of drugs and/or their metabolites. The kit was designed for screening purposes and is intended for forensic use only. It is recommended that all suspect samples be confirmed by a quantitative method such as gas chromatography/mass spectrometry (GC/MS).

Standard Curve