Cocaine/Benzoylecgonine-2 ELISA Kit

Sample

Urine, Blood, Oral Fluid, Breast Milk, Forensic Matrices

Species Reactivity

Human; Research Animal

Detection Method

cELISA

Intended Use

For the determination of trace quantities of Cocaine, Benzoylecgonine and/or other metabolites in human urine, blood, oral fluid.

Contents of Kit

1. EIA Buffer: 30 mL (ready-to-use). Phosphate buffered saline solution with bovine serum and a preservative. Provided for dilution of samples.
2. Wash Buffer Concentrate (10X): 20 mL. Phosphate buffered saline solution with a surfactant. Dilute 10 fold with deionized or ultrapure water before use. Diluted wash buffer is used to wash all unbound conjugate and samples from the plate after the conjugate incubation.
3. K-Blue Substrate: 20 mL (ready-to-use). Stabilized 3,3', 5,5' Tetramethylbenzidine (TMB) plus Hydrogen Peroxide (H₂O₂) in a single bottle. It is used to develop the color in the wells after washing. Light sensitive.
4. Drug-Enzyme Conjugate: 23 mL (ready-to-use). Drug-horseradish peroxidase concentrate. Do not dilute.
5. Antibody Coated Plate: A 96 well Costar plate, in strips of 8 break-away wells, coated with anti-drug antiserum. The plate is ready for use as is. Do not wash.
6. Acid Stop Solution: 14 mL (ready-to-use). 1 N H₂SO₄ used to stop the enzyme reaction.
7. Whole Blood Cut-off Calibrators: DO NOT DILUTE.
a. Blood Negative Calibrator: 1 mL provided in a synthetic matrix (red cap).
b. Blood Cut-off Calibrator (BZE): 1 mL provided in a synthetic matrix, 50 ng/mL (red cap).
8. Urine Cut-off Calibrators: DO NOT DILUTE.
a. Urine Negative Calibrator: 1 mL provided in a synthetic matrix (yellow cap).
b. Urine Cut-off Calibrator (BZE): 1 mL provided in a synthetic matrix, 300 ng/mL (yellow cap).

Storage

This kit can be used until the expiration date on the label when stored refrigerated at 2-8°C.

Sensitivity

The term I-50 is used to define the sensitivity of the test. This number is derived from a standard curve generated with the drug in EIA Buffer. The drug concentration that shows 50% less color activity than the zero standard is considered to be the I-50.

General Description

CD' s Cocaine/Benzoylecgonine-2 ELISA (Enzyme-Linked ImmunoSorbent Assay) test kit is a qualitative one-step kit designed for use as a screening device for the detection of drugs and/or their metabolites. The kit was designed for screening purposes and is intended for forensic use only. It is recommended that all suspect samples be confirmed by a quantitative method such as gas chromatography/mass spectrometry (GC/MS).

Standard Curve