Mouse Anti-ASFV p30 monoclonal antibody (CABT-RM035)

Mouse Anti-ASFV p30 monoclonal antibody for ELISA, IF, IHC, IP, WB

Specifications


Host Species
Mouse
Clone
D12993N
Species Reactivity
ASFV
Immunogen
p30 Recombinant Protein.
Conjugate
Unconjugated

Applications


Application Notes
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
ASFV phosphoprotein p30; African swine fever Virus; ASFV;ASFV p30

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


Exposure of extensively farmed wild boars (Sus scrofa scrofa) to selected pig pathogens in Greece

VETERINARY QUARTERLY

Authors: Marinou, K. A.; Papatsiros, V. G.; Gkotsopoulos, E. K.; Odatzoglou, P. K.; Athanasiou, L. V.

Background: Increased density and distribution of wild boar populations are likely to promote interactions and transmission of certain pathogens, not only among wild boar but also from wild boar to livestock or humans and vice versa. Objective: The purpose of this study was to determine seroprevalence against seven selected pathogens in wild boar living in four different areas in Greece. Animals and methods: In total, 359 serum samples were collected from extensively farmed wild boar (Sus scrofa scrofa) originating from four distinct geographical areas throughout Greece from April 2012 to August 2013. Samples were tested for antibodies to Actinobacillus pleuropneumoniae, African swine fever virus (ASFV), Aujeszky's disease virus (ADV), classical swine fever virus (CSFV), Erysipelothrix rhusiopathiae, Mycoplasma hyopneumoniae and porcine reproductive and respiratory syndrome virus (PRRSV). Prevalence was compared among the four regions using Fisher's exact test. Results: Low overall seropositivities of 2.4% and 5.6% were detected for E. rhusiopathiae and PRRSV, respectively, higher ones for ADV (32.0%) and the highest (72.5% and 90.5%) for M. hyopneumoniae and A. pleuropneumoniae, respectively. All sera tested were found negative for antibodies directed against CSFV and ASFV. Conclusions: This is the first report of exposure of wild boars to selected pig pathogens in Greece. These results are indicative of the circulation of these pathogens in Greece with the exception of CSFV and ASFV and suggestive of the potential role of wild boars on their maintenance and transmission to their domestic counterparts and vice versa.

Genome comparison of African swine fever virus China/2018/AnhuiXCGQ strain and related European p72 Genotype II strains

TRANSBOUNDARY AND EMERGING DISEASES

Authors: Bao, Jingyue; Wang, Qinghua; Lin, Peng; Liu, Chunju; Li, Lin; Wu, Xiaodong; Chi, Tianying; Xu, Tiangang; Ge, Shengqiang; Liu, Yutian; Li, Jinming; Wang, Shujuan; Qu, Hailong; Jin, Tao; Wang, Zhiliang

African swine fever was introduced into China in August 2018 and led to high mortality in domestic pigs. We reported the genome characterization of the China/2018/AnhuiXCGQ strain mainly based on next-generation sequencing and comparison with related European p72 Genotype II strains. The genome was 189,393 bp long, encoding 181 open reading frames. Pair-wise genome sequence comparison revealed 54-107 variation sites between China/2018/AnhuiXCGQ and the other genotype II virulent strains contributing to the change of expression or alteration of amino acid residues in 10-38 genes. China/2018/AnhuiXCGQ strain shared the highest similarity with POL/2015/Podlaskie strain. Phylogenetic analysis based on a 125 kb long conserved central region revealed that the China/2018/AnhuiXCGQ strain and four European genotype II strains were grouped into three clusters. This study expanded our knowledge on the genetic diversity and evolution of ASFV and provided valuable information for diagnosis improvement and vaccine development.

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