HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The VIM knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
Nature
Cell Lysate
Alternative Names
VIM; vimentin; HEL113; CTRCT30; epididymis luminal protein 113;
Application Notes
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Dilution
Lysate samples can be diluted with 2x SDS Sample Buffer. After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
Format
Lyophilized
Concentration
The protein concentration was determined with BCA assay.
Buffer
RIPA buffer
Preservative
None
Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.
Citations
Publication ()
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References
ANALYTIC APPROXIMATE SOLUTIONS FOR THE 1D AND 2D NONLINEAR FRACTIONAL DIFFUSION EQUATIONS OF FISHER TYPE
In this paper, analytical approximate solutions for solving the one dimensional (1D) and two dimensional (2D) fractional time diffusion equations of Fisher type (FTDEFT) are given via the Laplace variational iteration method (LVIM). The proposed method is a combination of two powerful methods; Laplace transform (LT) and variational iteration method (VIM). The achieved solutions are given as a speedily convergent series with simply computable terms. The suggested technique is effective and easy to implement. The proposed technique is tested by introducing three different examples. These examples include the 1D fractional time Fisher equation, the fractional time Burger- Fisher equation and the 2D generalized fractional time Fisher type equation. The obtained solutions demonstrate the accuracy of the proposed method when compared with the exact solutions or the numerical solutions in the existing literature. The conclusion of the study exposes that the LVIM is computationally very effective and accurate to analyze nonlinear fractional differential equations. Also with this method, the nature of results can be scanned when the fractional derivative factors are varied.
THE AGREEMENT BETWEEN THE NEW EXACT AND NUMERICAL SOLUTIONS OF THE 3D-FRACTIONAL WAZWAZ-BENJAMIN-BONA-MAHONY EQUATION
JOURNAL OF SCIENCE AND ARTS
Authors: Bekir, Ahmet; Zahran, Emad H. M.; Shehata, Maha S. M.
In this article, we employed the 3D-fractional Wazwaz-Benjamin-BonaMahony (3D-FWBBM) equation with its spatial and temporal variables which is stretch for the Korteweg-de-Vries equation that represent the unidirectional propagation of small amplitude long waves on the surface of hydro magnetic and acoustic waves in channel specially for shallow water. New exact soliton solution has been realized using the (G'/G)-expansion method. Furthermore, the numerical solution of the suggested equation according to the variational iteration method (VIM) is listed effectively. A good comparison between the obtained exact and numerical solution are successfully demonstrated.
COA
Publication 
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