Anti-VIM monoclonal antibody (DMAB5565RH)

Rabbit Anti-VIM monoclonal antibody for WB, ICC/IF, IHC, IP, FC Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
TD71-16
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:1,000-1:5,000; ICC: 1:100-1:500; IHC: 1:100-1:500; FC: 1:50-1:100
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of Vimentin on different lysates using anti-Vimentin antibody at 1/1,000 dilution. Positive control: Lane 1: Hela; Lane 2: A549
Fig10: Flow cytometric analysis of Hela cells with Vimentin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody
Fig2: ICC staining Vimentin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Vimentin in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Vimentin in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: ICC staining Vimentin in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Vimentin antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Vimentin antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Vimentin antibody. Counter stained with hematoxylin.
Fig9: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Vimentin antibody. Counter stained with hematoxylin.

Target


Alternative Names
CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; VIM; VIME_HUMAN

Citations


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References


Ottonello, L; Epstein, AL; et al. Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: Intervention of Fc gamma R-II(CD32), CD11b-CD18 integrins, and CD66b glycoproteins. BLOOD 93:3505-3511(1999).
Colace, TV; Jobson, J; et al. Relipidated Tissue Factor Linked to Collagen Surfaces Potentiates Platelet Adhesion and Fibrin Formation in a Microfluidic Model of Vessel Injury. BIOCONJUGATE CHEMISTRY 22:2104-2109(2011).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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