ExoAssay™ Tumor-derived Exosome enrichment and quantification ELISA Kit

Sample

plasma, serum, urine

Species Reactivity

Human

Intended Use

Exosomal Ready to Use Kit is the ideal platform for capturing and the quantifying exosomes from biological samples such as plasma, serum, urine and cell culture supernatant.
Exosomal Ready to Use Kit features pre-coated ELISA plate with primary antibodies detection directed against exosome-specific markers, namely α-CD9. Secondary antibodies and substrate reagents are included in the kit.
Exosome standards for assay calibration are also included in the kit. Exosome standards are purified by ultracentrifugation and microfiltration, quantified for overall protein content and exosome number and then lyophilized for long-term storage.

Storage

All the reagents are shipped at 4°C and storage conditions as recommended in the product insert.

Sensitivity

The detection limit of an assay is lower than 0.35 ug of overall exosome which is an equivalent of less than 50 pg of targeted exosomes protein.

General Description

Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell.
Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.