Toxoplasma gondii IgA Antibody ELISA Kit

Sample

Serum

Species Reactivity

Human

Detection Method

iELISA

Intended Use

The Toxoplasma gondii IgA Enzyme Immunoassay Kit provides materials for determination of IgA-class antibodies to Toxoplasma gondii in serum.

Contents of Kit

1. Microtiterwells, 12 x 8 (break apart) strips, 96 wells;
Wells coated with inactivated Toxoplasma gondii antigen.
(incl. 1 strip holder and 1 cover foil)
2. Sample Diluent ***, 1 vial, 100 mL, ready to use,
colored yellow; pH 7.2 ± 0.2.
3. IgG-RF-Sorbent***, 1 vial, 6.5 mL, ready to use,
colored yellow;
Contains anti-human IgG-class antibody.
4. Pos. Control ***, 1 vial, 2.0 mL, ready to use;
colored yellow, red cap.
5. Neg. Control ***, 1 vial, 2.0 mL, ready to use;
colored yellow, yellow cap.
6. Cut-off Control ***, 1 vial, 2.0 mL, ready to use;
colored yellow, black cap.
7. Enzyme Conjugate **, 1 vial, 20 mL, ready to use;
colored red,
antibody to human IgA conjugated to horseradish peroxidase.
8. Substrate Solution, 1 vial, 14 mL, ready to use;
Tetramethylbenzidine (TMB).
9. Stop Solution, 1 vial, 14 mL, ready to use;
contains 0.2 mol/l H₂SO₄.
Avoid contact with the stop solution. It may cause skin irritations and burns.
10. Wash Solution *, 1 vial, 30 mL (20X concentrated for 600 mL), pH 7.2 ± 0.2.
see "Preparation of Reagents".
* contains 0.03 % ProClin 300
** contains 0.03 % ProClin 300 + 0.01 % Gentamicin sulphate
*** contains 0.03 % ProClin 300 + 0.015 % 5-bromo-5-nitro-1,3-dioxane (BND) + 0.010 % 2-methyl-2H-isothiazol-3-one (MIT)

Storage

1. When stored at 2-8°C unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
2. Opened reagents must be stored at 2-8°C. Microtiter wells must be stored at 2-8°C. Once the foil bag has been opened, care should be taken to close it tightly again.
3. Opened kits retain activity for four months if stored as described above.