Molecular Weight
47.8 kDa
Alternative Names
T. gondii; T. gondii Mag1; Toxoplasma gondii Mag1; T. gondii Cyst matrix protein
Purity
98%, analysed by SDS-PAGE (reducing and non-reducing) followed by Coomassie-Blue staining and immunoprobing of Western blotted proteins with anti-E.coli serum (DAKO)
Buffer
Antigen specific solution, contains either 8M Urea + 2mM DTE or 0.1% SDS.
Storage
At -80°C 10 years from the date of production. Avoid repeated freeze-thaw cycles
Introduction
Toxoplasma gondii is a species of parasitic protozoa in the genus Toxoplasma. The definitive host of T. gondii is the cat, but the parasite can be carried by many warm-blooded animals (birds or mammals, including humans). Toxoplasmosis, the disease of which T. gondii is the causative agent, is usually minor and self-limiting but can have serious or even fatal effects on a fetus whose mother first contracts the disease during pregnancy or on an immunocompromised human or cat. Around a third of people worldwide carry the parasite, with most catching it by consuming undercooked meat, especially lamb, pork and venison or by ingesting water, soil or anything contaminated by feline faeces.
Antigen Description
Recombinant Toxoplasma gondii Mag1 is expressed in E. coli as a monomeric, non-glycosylated polypeptide chain consisting of 437 amino acids with a molecular mass of 47800 Da. The product represents the complete sequence of the mature Mag1, but lacks the putative signal sequence at the N-terminus of the native protein. It carries an N-terminal fusion of 6 Histidines.
Keywords
T. gondii; T. gondii Mag1; Toxoplasma gondii Mag1; T. gondii Cyst matrix protein
Citations
Publication ()
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