Rat TEK Tyrosine Kinase, Endothelial ELISA Kit

Name: Rat TEK Tyrosine Kinase, Endothelial ELISA Kit
SKU: DEIA-BJ2298
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Species Reactivity

Rat

Detection Method

Competitive ELISA

Intended Use

Rat TEK Tyrosine Kinase, Endothelial ELISA Kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of the TEK Tyrosine Kinase, Endothelial. This ELISA kit is for research use only, not for therapeutic or diagnostic applications.

Contents of Kit

1. MICROTITER PLATE: 96 wells
2. ENZYME CONJUGATE: 6.0 mL or 10 ml
3. STANDARD A-F: 1 vial each
4. SUBSTRATE A: 6 mL
5. SUBSTRATE B: 6 mL
6. STOP SOLUTION: 6 mL
7. WASH SOLUTION (100 x): 10 mL
8. BALANCE SOLUTION: 3 mL

Storage

All components of this kit are stable at 2-8°C until the kit's expiration date.

Detection Range

1.0-25 ng/mL

Sensitivity

0.1 ng/mL

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

OMNIBOT: A Robotic Verticalization Mobility System for People with Disabilities

IFAC PAPERSONLINE

Authors: Bakalova, Marina; Minina, Radostina

Abstract

In this paper we present an innovative Bulgarian robotic device OMNIBOT for enabling people with motor disabilities to move in stand-up position. First, we present the challenges and the state of art in the domain focusing on the competitive device TEK RMD (Matia Robotics). Then we present OMNIBOT with its technical characteristics and advantages. In the last part, we present the business plan of the product and we estimate the social and economic consequences of the potential mass production of this innovation. (c) 2019, IFAC (International Federation of Automatic Control) Hosting by Elsevier Ltd. All rights reserved.

Endothelial Basement Membrane Laminins as an Environmental Cue in Monocyte Differentiation to Macrophages

FRONTIERS IN IMMUNOLOGY

Authors: Li, Lixia; Song, Jian; Chuquisana, Omar; Hannocks, Melanie-Jane; Loismann, Sophie; Vogl, Thomas; Roth, Johannes; Hallmann, Rupert; Sorokin, Lydia

Abstract

Monocyte differentiation to macrophages is triggered by migration across the endothelial barrier, which is constituted by both endothelial cells and their underlying basement membrane. We address here the role of the endothelial basement membrane laminins (laminins 411 and 511) in this monocyte to macrophage switch. Chimeric mice carrying CX3CR1-GFP bone marrow were employed to track CCL2-induced monocyte extravasation in a cremaster muscle model using intravital microscopy, revealing faster extravasation in mice lacking endothelial laminin 511 (Tek-cre::Lama5(-/-)) and slower extravasation in mice lacking laminin 411 (Lama4(-/-)). CX3CR1-GFP(low) extravasating monocytes were found to have a higher motility at laminin 511 low sites and to preferentially exit vessels at these sites. However, in vitro experiments reveal that this is not due to effects of laminin 511 on monocyte migration mode nor on the tightness of the endothelial barrier. Rather, using an intestinal macrophage replenishment model and in vitro differentiation studies, we demonstrate that laminin 511, together with the attached endothelium, promote monocyte differentiation to macrophages. Macrophage differentiation is associated with a change in integrin profile, permitting differentiating macrophages to distinguish between laminin 511 high and low areas and to preferentially migrate across laminin 511 low sites. These studies highlight the endothelial basement membrane as a critical site for monocyte differentiation to macrophages, which may be relevant to the differentiation of other cells at vascular niches.