Anti-Human TEK (Phospho-Tyr1102) polyclonal antibody (CABT-L4144)

Rabbit Anti-Human TEK (Phospho-Tyr1102) polyclonal antibody for IHC, ELISA

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human, Mouse
Immunogen
The antiserum was produced against synthesized peptide derived from human TIE2 around the phosphorylation site of Tyr1102. Immunogen range: 1068-1117
Conjugate
Unconjugated

Applications


Application Notes
Recommended diltion:
IHC: 1:50~1:100; ELISA: 1:40000
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


IHC analysis of paraffin-embedded human brain, using TIE2 (Phospho-Tyr1102) Antibody. The picture is blocked with the phospho peptide.

Target


Alternative Names
TEK; TEK tyrosine kinase, endothelial; TIE2; VMCM; TIE-2; VMCM1
Entrez Gene ID
UniProt ID

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


Angiopoietin-1 deficiency increases tumor metastasis in mice

BMC CANCER

Authors: Michael, Iacovos P.; Orebrand, Martina; Lima, Marta; Pereira, Beatriz; Volpert, Olga; Quaggin, Susan E.; Jeansson, Marie

Background: Angipoietin-1 activation of the tyrosine kinase receptor Tek expressed mainly on endothelial cells leads to survival and stabilization of endothelial cells. Studies have shown that Angiopoietin-1 counteracts permeability induced by a number of stimuli. Here, we test the hypothesis that loss of Angiopoietin-1/Tek signaling in the vasculature would increase metastasis. Methods: Angiopoietin-1 was deleted in mice just before birth using floxed Angiopoietin-1 and Tek mice crossed to doxycycline-inducible bitransgenic ROSA-rtTA/tetO-Cre mice. By crossing Angiopoietin-1 knockout mice to the MMTV-PyMT autochthonous mouse breast cancer model, we investigated primary tumor growth and metastasis to the lung. Furthermore, we utilized B16F10 melanoma cells subcutaneous and experimental lung metastasis models in Angiopoietin-1 and Tek knockout mice. Results: We found that primary tumor growth in MMTV-PyMT mice was unaffected, while metastasis to the lung was significantly increased in Angiopoietin-1 knockout MMTV-PyMT mice. In addition, angiopoietin-1 deficient mice exhibited a significant increase in lung metastasis of B16F10 melanoma cells, compared to wild type mice 3 weeks after injection. Additional experiments showed that this was likely an early event due to increased attachment or extravasation of tumor cells, since seeding of tumor cells was significantly increased 4 and 24 h post tail vein injection. Finally, using inducible Tek knockout mice, we showed a significant increase in tumor cell seeding to the lung, suggesting that Angiopoietin-1/Tek signaling is important for vascular integrity to limit metastasis. Conclusions: This study show that loss of the Angiopoietin-1/Tek vascular growth factor system leads to increased metastasis without affecting primary tumor growth.

DYNAMIC ECOLOGICAL KNOWLEDGE SYSTEMS AMID CHANGING PLACE AND CLIMATE: MT. YULONG RHODODENDRONS

JOURNAL OF ETHNOBIOLOGY

Authors: Hart, Robbie; Salick, Jan

How dynamic is place-based traditional ecological knowledge (TEK)? In what ways can its generative and conservative processes allow adaption to a changed environment? How do different cultures mediate TEK of a shared place? We address these questions with an in depth study of TEK of the diverse and socioecologically salient genus Rhododendron among the indigenous Naxi and immigrant Nuosu Yi of Mt. Yulong, NW Yunnan, China. TEK in both cultures is rich and intimately connected to the seasonal and elevational progression of rhododendrons. Naxi and Yi knowledge of trends and drivers of change parallel those in ecological studies. Knowledge richness was connected with place (urban vs. rural dwelling and elevation of village) and the immigrant Yi had a knowledge base as rich as that of the indigenous Naxi. Both Yi and Naxi interviewees credited this knowledge equality to a combination of generative processes (Yi villages were higher in elevation and Yi livelihoods made more use of mountain resources, which enabled them to acquire knowledge of plants quickly) and conservative processes (Yi migrated from an equally diverse mountain region in which Rhododendron is also salient; its position was retained in their system of TEK, though its elements were adapted). Among rural Naxi, cultural systems (seasonal festivals and ethnotaxonomy) conserved knowledge, even while their direct use of rhododendrons decreased with changing life-ways.

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