Anti-RSPaV polyclonal antibody (CABT-B1007)

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Rupestris Stem Pitting Associated Virus
Immunogen
A mixture of three synthetic peptides corresponding to aa 7-19 (KLPGESNEAFEAR), aa 112-125 (SDVGSSQRSTLIGK), and aa 232-244 (KRSSNLGEISGGT) of the RSPaV coat protein
Conjugate
Unconjugated

Applications


Application Notes
WB: 1:500
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
Rupestris Stem Pitting Associated Virus

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


Molecular analysis of a California strain of Rupestris stem pitting-associated virus isolated from declining Syrah grapevines

ARCHIVES OF VIROLOGY

Authors: Lima, M. F.; Alkowni, R.; Uyemoto, J. K.; Golino, D.; Osman, F.; Rowhani, A.

The sequence of the genome of a Rupestris stem pitting-associated virus (RSPaV) isolated from a declining Syrah grapevine in California, designated the Syrah strain (RSPaV-SY) was determined. The genome of this strain had an overall nucleotide identity of 77% in comparison with RSPaV sequences in GenBank; the coat protein was the most conserved gene among RSPaV sequences and the replicase was the least conserved gene. Phylogenetic analysis of partial coat protein and replicase gene sequences showed RSPaV-SY clustered independently from the majority of RSPaV isolates.

Survey for viruses of grapevine in Oregon and Washington

PLANT DISEASE

Authors: Martin, RR; Eastwell, KC; Wagner, A; Lamprecht, S; Tzanetakis, IE

Grapevines (Vitis spp.) in Washington and Oregon were surveyed for the prevalence of key grapevine viruses. Samples collected from 1,522 vines in Washington were tested for Rupestris stem pitting associated virus (RSPaV), Grapevine fanleaf virus (GFLV), Arabis mosaic virus (ArMV), Tomato ringspot virus (ToRSV), and Grapevine leafroll associated virus-3 (GLRaV-3). Tests were also conducted for GLRaV-1 and -2 on 420 samples from Washington. Two hundred forty samples collected from wine grape vineyards in Oregon were tested for GLRaV-1, -2, and -3, and an additional 2,880 samples were collected from 40 vineyards known to have high populations of Xiphinema americanum nematodes. The latter were tested for ArMV, TbRSV, and GFLV. GLRaV-1, -2, and -3 were detected in 2.6, 0.2, and 6.5% of the Washington sample, and in 3.0, 0.4, and 4.4% of the Oregon samples. RSPaV was detected in 4.6% of the samples from Washington. No ToRSV. ArMV, or GFLV was detected in any of the samples from Oregon or Washington. Transmission of field isolates of GLRaV-3 from Washington by the grape mealybug also was demonstrated.

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