Grapevine Rupestris Stem Pitting-associated Virus (GRSPaV) ELISA Kit

Name: Grapevine Rupestris Stem Pitting-associated Virus (GRSPaV) ELISA Kit
SKU: DEIAPV164
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

host plants

Intended Use

The test can be used to detect GRSPaV in infected host plant tissues.

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is high. The virus can be consistently detected in infected plant tissues diluted at 1:30 -1:270.

General Description

Grapevine Rupestris stem pitting-associated virus (GRSPaV) is a member of the genus Foveavirus within the family Flexiviridae. GRSPaV is closely associated with the disease Rupestris stem pitting and is frequently detected in grapevines worldwide.

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Deep sequencing analysis of viral short RNAs from an infected Pinot Noir grapevine

VIROLOGY

Authors: Pantaleo, Vitantonio; Saldarelli, Pasquale; Miozzi, Laura; Giampetruzzi, Annalisa; Gisel, Andreas; Moxon, Simon; Dalmay, Tamas; Bisztray, Gyoergy; Burgyan, Jozsef

Abstract

Virus-derived short interfering RNAs (vsiRNAs) isolated from grapevine V. vinifera Pinot Noir clone ENTAV 115 were analyzed by high-throughput sequencing using the Illumina Solexa platform. We identified and characterized vsiRNAs derived from grapevine field plants naturally infected with different viruses belonging to the genera Foveavirus, Maculavirus, Marafivirus and Nepovirus. These vsiRNAs were mainly of 21 and 22 nucleotides (nt) in size and were discontinuously distributed throughout Grapevine rupestris stem-pitting associated virus (GRSPaV) and Grapevine fleck virus (GFkV) genomic RNAs. Among the studied viruses, GRSPaV and GFkV vsiRNAs had a 5' terminal nucleotide bias, which differed from that described for experimental viral infections in Arabidopsis thaliana. VsiRNAs were found to originate from both genomic and antigenomic GRSPaV RNA strands, whereas with the grapevine tymoviruses GFkV and Grapevine Red Globe associated virus (GRGV), the large majority derived from the antigenomic viral strand, a feature never observed in other plant-virus interactions. (C) 2010 Elsevier Inc. All rights reserved.

Grapevine virus T diversity as revealed by full-length genome sequences assembled from high-throughput sequence data

PLOS ONE

Authors: Zarghani, Shaheen Nourinejhad; Hill, Jean Michel; Glasa, Miroslav; Marais, Armelle; Wetzel, Thierry; Faure, Chantal; Vigne, Emmanuelle; Velt, Amandine; Lemaire, Olivier; Boursiquot, Jean Michel; Okic, Arnela; Belen Ruiz-Garcia, Ana; Olmos, Antonio; Lacombe, Thierry; Candresse, Thierry

Abstract

RNASeq or double-stranded RNA based approaches allowed the reconstruction of a total of 9 full-length or near full-length genomes of the recently discovered grapevine virus T (GVT). In addition, datamining of publicly available grapevine RNASeq transcriptome data allowed the reconstruction of a further 14 GVT genomes from five grapevine sources. Together with four GVT sequences available in Genbank, these novel sequences were used to analyse GVT diversity. GVT shows a very limited amount of indels variation but a high level of nucleotide and aminoacid polymorphism. This level is comparable to that shown in the closely related grapevine rupestris stem pitting-associated virus (GRSPaV). Further analyses showed that GVT mostly evolves under conservative selection pressure and that recombination has contributed to its evolutionary history. Phylogenetic analyses allow to identify at least seven clearly separated groups of GVT isolates. Analysis of the only reported PCR GVT-specific detection primer pair indicates that it is likely to fail to amplify some GVT isolates. Taken together these results point at the distinctiveness of GVT but also at the many points it shares with GRSPaV. They constitute the first pan-genomic analysis of the diversity of this novel virus.