U1-RNP-Ab ELISA Kit (DEIABL379)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum
Species Reactivity
Human
Intended Use
Enzyme immunoassay for the qualitative and quantitative determination of IgG antibodies against U1-RNP in human serum.
Contents of Kit
Microtiter Plate: 1 x 12 x 8
Enzyme Conjugate IgG: 1 x 14 mL
Calibrator A - F: 1 x 6 x 2 mL
Positive Control: 1 x 2 mL
Negative Control: 1 x 2 mL
Sample Diluent: 1 x 100 mL
TMB Substrate Solution: 1 x 14 mL
Wash Buffer, Concentrate (10x): 1 x 100 mL
TMB Stop Solution: 1 x 14 mL
Storage
2-8°C
Precision
Detection Range
0 - 60 U/mL, cut-off 4 U/mL
Sensitivity
0.5 U/mL
General Description
Enzyme immunoassay for the qualitative and quantitative determination of IgG antibodies against U1-RNP in human serum. Uracil-rich, small nuclear RNAs (U-snRNA) are physiologically complexed with proteins to ribonucleoprotein particles (U-snRNP) which are involved in the splicing process of pre-mRNA. Different complexes (U1-U6) can be distinguished according to the participating RNA; their protein components are in part common, in part different. For example, U1-snRNA is complexed with the so-called Sm-proteins which are also present in other U-snRNPs, and with the U1-specific proteins A, C and 68 kDa. Autoantibodies directed against these three U1-specific proteins are considered as diagnostic marker for mixed connective tissue disease (MCTD). They are also associated with systemic lupus erythematosus (SLE) but occur with distinctly lower prevalence (about 35%) and mostly in combination with further antinuclear antibodies (ANA). Nowadays, proteins A, C and 68 kDa are regarded as the actual RNP antigen, rather than the complete U1-RNP.
Standard Curve

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