Potato virus Y-necrotic strain (PVY-n) ELISA Kit

Name: Potato virus Y-necrotic strain (PVY-n) ELISA Kit
SKU: DEIAPV236
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Host plants

Species Reactivity

Host plants

Detection Method

sELISA

Intended Use

This ELISA test is a qualitative serological assay for the detection of Potato virus Y - necrotic strain (PVY-n) in potato leaves and sprouts. PVY-n is a member of the Potyvirus genus known for their non-enveloped, flexuous, filamentous virus particles. This test also detects the PVY-NTN strains that cause the tuber necrotic ringspot disease (PTNRD) of potatoes.

Contents of Kit

1. Capture antibody
2. Detection antibody, bottle A
3. Alkaline phosphatase enzyme conjugate, bottle B
4. 96-well microtiter plates, solid* (*strip in single plate set)
Buffer Pack:
1. Carbonate coating buffer, 10× concentrate
2. PNP substrate buffer, 5× concentrate
3. PNP substrate tablets, 5 mg each
4. PBST buffer, powder
5. Nonfat Dried Milk

Storage

Antibodies should be stored refrigerated (2 - 8 °C) between uses. All test materials should be warmed to room temperature (18 - 30 °C) before use.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Detection of molecular markers linked to Ry genes in potato germplasm for marker-assisted selection for extreme resistance to PVY in AAFC's potato breeding program

CANADIAN JOURNAL OF PLANT SCIENCE

Authors: Nie, Xianzhou; Lalany, Fayruza; Dickison, Virginia; Wilson, Donna; Singh, Mathuresh; De Koeyer, David; Murphy, Agnes

Abstract

Molecular markers reported to be linked to extreme resistance (ER) against Potato virus Y (PVY) were evaluated in potato germplasm. YES3-3A and YES3-3B, markers linked to Ry(sto), were detected in 'Barbara' and its three descendants that exhibit ER to PVY; RYSC3, a marker linked to Ry(adg), was detected in breeding clones NY121 and NY123. Assessment of RYSC3 as a marker for selection for Ry(adg)-mediated ER validated its efficacy in identification of selections with ER to PVY.

'BRS Clara': A late-blight-resistant fresh market potato cultivar

HORTICULTURA BRASILEIRA

Authors: Pereira, Arione da S.; Bertoncini, Odone; da Silva, Giovani O.; Castro, Caroline M.; Gomes, Cesar B.; Hirano, Elcio; Bortoletto, Antonio Cesar; de Melo, Paulo Eduardo; Medeiros, Carlos Alberto B.; Treptow, Rosa O.; Dutra, Leonardo F.; Lopes, Carlos Alberto; Nazareno, Nilceu R. X.; Lima, Mirtes F.; de Castro, Luis Antonio S.; Krolow, Ana Cristina R.; Suinaga, Fabio A.; Reisser Junior, Carlos

Abstract

'BRS Clara' is a new potato cultivar for the fresh market, released in 2010. It was developed by the Potato Breeding Program of Embrapa, selected for tuber appearance and yield, and foliar resistance to late blight caused by Phytophthora infestans. The tubers have yellow and smooth skin, cream colored flesh, elongated-oval shape and shallow eyes. It has high yield potential. In the South region of Brazil, 'BRS Clara' yielded higher or not different from Agata cultivar, and did not differ from 'Asterix'. In the autumn season, it yielded higher than 'Agata' for total and marketable tubers. This superiority was related to late blight resistance of 'BRS Clara'. In the spring season, it did not differ from 'Agata' and 'Asterix'. The specific gravity of 'BRS Clara' was higher than of 'Agata', and lower than of 'Asterix'. 'BRS Clara' had low incidence of physiological defects. In relation to the main culinary uses, it was rated as regularly good to good for salad, lightly good for baking, and varied from poor to good for French fries. The resistance to greening was moderate. It showed moderate to high resistance to late blight. Field observations indicate that 'BRS Clara' has moderate resistance to early blight, and susceptibility to mosaic (PVY) and leafroll (PLRV).