The test can be used to detect PVY in infected potato leaves, tuber eyes, sprouts, and stems and other host plants.
Contents of Kit
1. Coating Antibody: 0.25 mL. Specific capture polyclonal antibody (PAb) or monoclonal antibody (MAb) for coating ELISA plate. 2. Detecting Conjugate A: 0.25 mL. 3. Detecting Conjugate B: 0.25 mL. 4. ELISA Plate: 5×96T. Empty ELISA plate with high binding capacity. The plate should be used for our ELISA Reagents to achieve a maximum test results. 5. Coating Buffer 10×: 6 mL 6. Sample Buffer: 11.6g 7. Conjugate Buffer 10×: 6 mL 8. PBST Buffer (Powder): 28.7g 9. Tween-20: 7.0g 10. PNP Buffer (5×): 10 mL 11. PNP Tablet: 10 Tablets
Storage
Store all buffers at 2-8°C, except for PNP tablets at -20°C under dark.
General Description
PVY is one of the most prevalent and important viruses in potatoes, which causes significant yield losses. It is transmitted mechanically and by aphids in a nonpersistent manner. The best strategy to control PVY is to use seed potatoes certified to have low virus content.
Citations
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Background
Potato virus Y (PVY) is the type species of the genus Potyvirus, one of the largest groups of plant viruses, with about 160 species. PVY is widely distributed throughout the world and infects many economically important plant species. These include potato, tobacco, tomato and pepper. Different strains of PVY show different symptoms on different parts of the host plant, and the host range of PVY includes 495 species of plants of 31 families and 72 genera, and PVY has several strains and has become one of the most economically important pathogens and one of the most important viruses of potato. PVY is transmitted from plant to plant in a non-persistent manner by more than 40 species of aphids and, in potato, by planting contaminated seed tubers. Potato plants infected with PVY will show different symptoms, with symptom expression and severity depending on potato variety, PVY strain, environmental conditions, and type of infection.
Figure 1. Main symptoms induced by Potato virus Y (PVY) in potato (Source: Karasev AV, et al. 2013)
In the past, people checked crops for disease by visual inspection, but visual inspection can easily overlook problems because symptoms are mild or the virus is in latent stage. Subsequently, with the development of technology, immunization or molecular testing of crops has replaced the visual inspection method. PVY can be detected in potato leaf or tuber samples by serological and RNA-based molecular assays. The ELISA currently in use detects the virus from leaves or tubers based on a basic double antibody sandwich assay. However, ELISA testing has been found to be unsatisfactory on dormant tubers and more reliable when tuber dormancy is broken by Rindite treatment. The PCR assay is more sensitive than traditional ELISA methods, detects low viral loads, and allows for multiple testing and differential diagnosis. However, it is more technically demanding and costly, compared to ELISA, which is more mature and is currently the commonly used screening method.
Here Creative Diagnostics provides you with an ELISA kit for the detection of PVY in infected potato leaves, tuber eyes, sprouts, stems and other host plants, which is highly sensitive and helps you to detect the virus infection in a timely manner.
Alternative Names
PVY ELISA Kit
References
1. Torrance L, et al. Potato Virus Y Emergence and Evolution from the Andes of South America to Become a Major Destructive Pathogen of Potato and Other Solanaceous Crops Worldwide. Viruses. 2020 Dec 12;12(12):1430
2. Karasev AV, et al. Continuous and emerging challenges of Potato virus Y in potato. Annu Rev Phytopathol. 2013;51:571-86.
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References
Cas13a-based multiplex RNA targeting for potato virus Y
Planta
Authors: Zhan X, Tu Z, Song W, Nie B, Li S, Zhang J, Zhang F.
The Cas13a-based multiplex RNA targeting system can be engineered to confer resistance to RNA viruses, whereas the number and expression levels of gRNAs have no significant effect on viral interference. The CRISPR-Cas systems provide adaptive immunity to bacterial and archaeal species against invading phages and foreign plasmids. The class 2 type VI CRISPR/Cas effector Cas13a has been harnessed to confer the protection against RNA viruses in diverse eukaryotic species. However, whether the number and expression levels of guide RNAs (gRNAs) have effects on the efficiency of RNA virus inhibition is unknown. Here, we repurpose CRISPR/Cas13a in combination with an endogenous tRNA-processing system (polycistronic tRNA-gRNA) to target four genes of potato virus Y (PVY) with varying expression levels. We expressed Cas13a and four different gRNAs in potato lines, and the transgenic plants expressing multiple gRNAs displayed similar suppression of PVY accumulation and reduced disease symptoms as those expressing a single gRNA. Moreover, PTG/Cas13a-transformed plants with different expression levels of multiple gRNAs displayed similar resistance to PVY strains. Collectively, this study suggests that the Cas13a-based multiplex RNA targeting system can be utilized to engineer resistance to RNA viruses in plants, whereas the number and expression levels of gRNAs have no significant effect on CRISPR/Cas13a-mediated viral interference in plants
Natural resistance to Potato virus Y in Solanum tuberosum Group Phureja
Theor Appl Genet
Authors: Torrance L, Cowan GH, McLean K, MacFarlane S, Al-Abedy AN, Armstrong M, Lim TY, Hein I, Bryan GJ.
Novel major gene resistance against Potato virus Y in diploid populations of Solanum tuberosum Groups Phureja and Tuberosum was biologically and genetically characterised. Named Ry(o)phu, it mapped to chromosome 9. A new source of genetic resistance derived from Solanum tuberosum Group Phureja against Potato virus Y (PVY) was identified and genetically characterised in three diploid biparental potato populations. Segregation data for two populations (05H1 and 08H1) suggested the presence of a single dominant gene for resistance to PVY which, following DaRT analysis of the 08H1 cross, was mapped to chromosome 9. More detailed genetic analysis of resistance utilised a well-characterised SNP-linkage map for the 06H1 population, together with newly generated marker data. In these plants, which have both S. tuberosum Group Phureja and S. tuberosum Group Tuberosum in their pedigree, the resistance was shown to map to chromosome 9 at a locus not previously associated with PVY resistance, although there is evidence for at least one other genetic factor controlling PVY infection. The resistance factor location on chromosome 9 (named as Ry(o)phu) suggests a potential role of NB-LRR genes in this resistance. Phenotypic analysis using a GUS-tagged virus revealed that a small amount of PVY replication occurred in occasional groups of epidermal cells in inoculated leaves of resistant plants, without inducing any visible hypersensitive response. However, the virus did not enter the vascular system and systemic spread was completely prevented.
COA
Publication 
Figure 1. Main symptoms induced by Potato virus Y (PVY) in potato
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