PTH-RP ( 1-34 ) ( human, rat, mouse ) ELISA Kit

Name: PTH-RP ( 1-34 ) ( human, rat, mouse ) ELISA Kit
SKU: DEIA2034
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

plasma

Species Reactivity

Human; Mouse; Rat

Detection Method

Competitive ELISA

Intended Use

This Enzyme Immunoassay kit is designed to detect a specific peptide and its related peptides based on the principle of competitive enzyme immunoassay.

Contents of Kit

1. 20X assay buffer concentrate ( 50 mL )
2. 96 well immunoplate ( 1 plate )
3. Acetate plate sealer ( APS ), ( 3 pieces ).
4. Primary antiserum ( rabbit anti-peptide IgG ) ( 1 vial )
5. Standard peptide ( 1 vial )
6. Biotinylated peptide ( 1 vial )
7. Streptavidin-horseradish peroxidase ( SA-HRP ) ( 30 μL )
8. Positive Control ( 2 vials )
9. Substrate solution TMB ( 12 mL )
10. 2N HCI ( 15 mL )

Storage

Store the kit at 4°C upon receipt. Store 1X assay buffer at 4°C. Remove any unneeded strips from antibody-coated plate, reseal them in zip lock foil and keep at 4°C. Keep rehydrated solution of Standard, Biotinylated peptide, Antibody and HRP at 4°C.

Detection Range

0-100 ng/mL

Citations

Have you cited DEIA2034 in a publication? Let us know and earn a reward for your research.

PTHrP intracrine actions divergently influence breast cancer growth through p27 and LIFR

Courtney M. Edwards, Jeremy F. Kane, Jailyn A. Smith, Déja M. Grant, Jasmine A. Johnson, Maria A. Hernandez Diaz, Lawrence A. Vecchi III, Kai M. Bracey, Tolu N. Omokehinde, Joseph R. Fontana, Breelyn A. Karno, Halee T. Scott, Carolina J. Vogel, Jonathan W. Lowery, T. John Martin & Rachelle W. Johnson

Breast Cancer Research2024 FebPubMed ID: 38409028Read Article

Applications: ELISA
Reactive species: Human
"Abstract: The role of parathyroid hormone (PTH)-related protein (PTHrP) in breast cancer remains controversial, with reports of PTHrP inhibiting or promoting primary tumor growth in preclinical studies. Here, we provide insight into these conflicting findings by assessing the role of specific biological domains of PTHrP in tumor progression through stable expression of PTHrP (-36-139aa) or truncated forms with deletion of the nuclear localization sequence (NLS) alone or in combination with the C-terminus. Although the full-length PTHrP molecule (-36-139aa) did not alter tumorigenesis, PTHrP lacking the NLS alone accelerated primary tumor growth by downregulating p27, while PTHrP lacking the NLS and C-terminus repressed tumor growth through p27 induction driven by the tumor suppressor leukemia inhibitory factor receptor (LIFR). Induction of p27 by PTHrP lacking the NLS and C-terminus persisted in bone disseminated cells, but did not prevent metastatic outgrowth, in contrast to the primary tumor site. These data suggest that the PTHrP NLS functions as a tumor suppressor, while the PTHrP C-terminus may act as an oncogenic switch to promote tumor progression through differential regulation of p27 signaling."
Article snippet: Undiluted conditioned media was added to 96-well ELISA plates to measure secreted PTHrP levels according to the manufacturer’s protocol (Creative Diagnostics, Catalog No. DEIA2034).

Secreted PTHrP (1-34aa) levels measured by ELISA from conditioned media of cells described in (A). (B-E & G) n = 3 independent biological replicates. Graphs represent mean ± SEM. (Courtney M. Edwards, <i>et al</i>, 2024)

Figure 1. Secreted PTHrP (1-34aa) levels measured by ELISA from conditioned media of cells described in (A). (B-E & G) n = 3 independent biological replicates. Graphs represent mean ± SEM.

Background

Parathyroid hormone-related protein (PTHrP) is a proteinaceous hormone that belongs to the parathyroid hormone (PTH) family. PTHrP is primarily secreted by mesenchymal stem cells, but it can also be produced by certain cancer cells, such as breast cancer and certain types of lung cancer. PTHrP plays a crucial role in regulating various physiological processes in the body. It acts as a versatile hormone, functioning as an endocrine, autocrine, paracrine, and intracrine signaling molecule. Its functions extend to different tissues and organs, including bone, teeth, vascular tissues, and mammary glands.

One of the key roles of PTHrP is in the regulation of bone development. It maintains the endochondral growth plate, which is responsible for longitudinal bone growth, at a constant width. PTHrP also influences epithelial-mesenchymal interactions during the formation of mammary glands, contributing to normal mammary gland development. Furthermore, PTHrP is involved in calcium homeostasis, a vital process in vertebrates. It helps regulate the levels of calcium in the body, ensuring its proper balance. PTHrP also has diverse functions in other systems. In the cardiovascular system, it acts as a vascular relaxant by affecting the smooth muscle of blood vessels. It also has variable effects on the heart, influencing its contractility over time. In the pancreas, PTHrP is involved in promoting the proliferation of β cells, which produce insulin, and inhibiting the apoptosis of these cells. In the central nervous system, PTHrP has a protective effect on neurons by safeguarding them from damage caused by excessive depolarization.

PTHrP is involved in the "cytokines excess" syndrome observed in cancer patients. It contributes to cancer cachexia by activating a gene program related to thermogenesis in brown adipose tissue. Unfortunately, blocking excessive cytokines or the PTH/PTHrP receptor does not alleviate cancer cachexia due to potential widespread side effects. However, the use of PTHrP as a reliable tumor marker in oncology is hindered by issues related to assay specificity, sensitivity, and analytical precision. Resolving these problems is crucial, especially considering that the function of PTHrP fragments formed by local proteolysis remains largely unknown.

Alternative Names

PTHLH (1-34) (human, rat, mouse) ELISA
PTH-rP (1-34) (human, rat, mouse) ELISA
PTH-RP ( 1-34 ) (human, rat, mouse) ELISA
PTH-related protein (1-34) (human, rat, mouse) ELISA
Parathyroid hormone-related protein (human, rat, mouse) ELISA
Parathyroid hormone-like hormone (human, rat, mouse) ELISA
PTHLH (1-34) (human, rat, mouse) ELISA Kit
PTH-rP (1-34) (human, rat, mouse) ELISA Kit
PTH-related protein (1-34) (human, rat, mouse) ELISA Kit
Parathyroid hormone-related protein (human, rat, mouse) ELISA Kit
Parathyroid hormone-like hormone (human, rat, mouse) ELISA Kit

Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Q & A

Ask a Question

Q: PTH-RP ( 1-34) ( human, rat, mouse) ELISA Kit (DEIA2034). There is also another ELISA kit for PTH 1-34 (DEIABL239). What is the difference between the two kits?

A: The detection targets are different.
DEIA2034: Detection of PTH-RP, which is a paracrine/autocrine/intracrine factor widely produced by many tissues of the body (breast, skin, blood vessels, placenta, fetal tissue, etc.). It plays many important roles in normal physiology, such as regulating chondrocyte differentiation (fetal bone development, growth plate), breast development, vascular tension, tooth eruption, skin hair growth, etc. It is not the main hormone for maintaining blood calcium homeostasis.
DEIABL239: Detection of PTH, which is synthesized and secreted by parathyroid chief cells. It is the main hormone for maintaining blood calcium homeostasis. Hypocalcemia stimulates its secretion, acting on bones and kidneys to increase blood calcium. Teriparatide is a synthetic drug (recombinant human PTH (1-34)). Its existence and function are entirely for therapeutic purposes (mainly osteoporosis).

Customer Reviews

References

PTHrP and skeletal development

Annals of the New York Academy of Sciences

Authors: Kronenberg H M.

Abstract

Parathyroid hormone-related protein (PTHrP) participates in the regulation of endochondral bone development. After the cartilage mold is established in fetal life, perichondrial cells and chondrocytes at the ends of the mold synthesize PTHrP. This ligand then acts on PTH/PTHrP receptors on chondrocytes. As chondrocytes go through a program of proliferation and then further differentiation into post-mitotic, hypertrophic chondrocytes, PTHrP action keeps chondrocytes proliferating and delays their further differentiation. Indian hedgehog (Ihh) is synthesized by chondrocytes that have just stopped proliferating and is required for synthesis of PTHrP. The feedback loop between PTHrP and Ihh serves to regulate the pace of chondrocyte differentiation and the sites at which perichondrial cells first differentiate into osteoblasts. Activation of the PTH/PTHrP receptor leads to stimulation of both Gs and Gq family heterotrimeric G proteins. Genetic analyses demonstrate that Gs activation mediates the action of PTHrP to keep chondrocytes proliferating, while Gq activation opposes this action. Downstream from Gs activation, synthesis of the cyclin-cdk inhibitor, p57, is suppressed, thereby increasing the pool of proliferating chondrocytes. PTHrP's actions to delay chondrocyte differentiation are mediated by the phosphorylation of the transcription factor, SOX9, and by suppression of synthesis of mRNA encoding the transcription factor, Runx2. These pathways and undoubtedly others cooperate to regulate the pace of differentiation of growth plate chondrocytes in response to PTHrP.

Twenty‐five years of PTHrP progress: From cancer hormone to multifunctional cytokine

Journal of Bone and Mineral Research

Authors: McCauley L K, Martin T J.

Abstract

Twenty-five years ago a "new" protein was identified from cancers that caused hypercalcemia. It was credited for its ability to mimic parathyroid hormone (PTH), and hence was termed parathyroid hormone-related protein (PTHrP). Today it is recognized for its widespread distribution, its endocrine, paracrine, and intracrine modes of action driving numerous physiologic and pathologic conditions, and its central role in organogenesis. The multiple biological activities within a complex molecule with paracrine modulation of adjacent target cells present boundless possibilities. The protein structure of PTHrP has been traced, dissected, and deleted comprehensively and conditionally, yet numerous questions lurk in its past that will carry into the future. Issues of the variable segments of the protein, including the enigmatic nuclear localization sequence, are only recently being clarified. Aspects of PTHrP production and action in the menacing condition of cancer are emerging as dichotomies that may represent intended temporal actions of PTHrP. Relative to PTH, the hormone regulating calcium homeostasis, PTHrP "controls the show" locally at the PTH/PTHrP receptor throughout the body. Great strides have been made in our understanding of PTHrP actions, yet years of exciting investigation and discovery are imminent.