Nitrofurazone (SEM) ELISA Kit

Sample

Feed, fish, shrimp, meat, honey, milk, serum, urine

Intended Use

This ELISA Kit provides a competitive enzyme immunoassay for the quantitative analysis of nitrofurazone in feed, fish, shrimp, meat, honey, milk, serum and urine.

Storage

2-8°C

Detection Range

The ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards).

Detection Limit

Fish&Shrimp: 0.05 ppb; Meat, Liver&Kidney: 0.05 ppb; Feed: 0.1 ppb; Egg&Egg Powder: 0.05 ppb; Milk&Honey: 0.05 ppb; Serum&Urine: 0.05 ppb

General Description

Nitrofurazone belongs to the class of 5-nitrofuran antibiotics and has been widely and effectively used for the prevention and treatment of gastrointestinal infections caused by Escherichia coli and Salmonella spp. in cattle, pigs and poultry. It was also used as a growth promoter in food-producing animals. However, both WHO and the European Union (EU) are unable to assign a maximum residue limit for nitrofurazone because of the potential carcinogenic effects of its residues on human health. As a consequence, the administration of nitrofurazone to food-producing animals has been prohibited. Because of the rapid excretion of the nitrofurans and their instability in vitro and in vivo, it is impossible to monitor residues of the parent drug nitrofurazone directly. Alternatively, semicarbazide (SEM), the major metabolite of nitrofurazone, which is stable in tissue, even after long-term storage, is selected to monitor.

Reconstitution And Storage

The shelf life is 12 months when the kit is properly stored.