EMSA: A representative lot caused a supershift of DNA-NRF-1 complex in EMSA using radiolabeled oligonucleotide containing human mitochondrial transcription specificity factor genes (TFB1M & TFB2M) NRF-1-binding site and HeLa nuclear extract (NE) or purified recombinant NRF-1. WB: An 1:200 dilution of this antibody detected NRF1 in 10 μg of HeLa nuclear extract.
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