NF-kappaB p100 (Phospho-Ser872) ELISA Kit

Name: NF-kappaB p100 (Phospho-Ser872) ELISA Kit
SKU: DEIA-XYA1317
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

nuclear lysates, cell lysates

Species Reactivity

Human; Mouse; Rat

Intended Use

The NF-kappaB p100 (Phospho-Ser872) DNA-Binding ELISA Kit contains components necessary for qualitative detection of active transcription factor NF-kappaB p100 (Phospho-Ser872) in eukaryotic nuclear or cell lysates.

Contents of Kit

1. 96-Well dsDNA Oligonucleotide Coated Microplate, 12 x 8-Well Microstrips.
2. 100x Primary Antibody, 100 μL, Yellow.
3. 100x Primary Phosho-Antibody, 100 μL, Orange.
4. HRP-Conjugated Anti-Rabbit IgG Antibody, 2x 6 mL, Amber.
5. Nuclear Lysate Positive Control, Lyophilized, Red.
6. Wild-Type Consensus dsDNA Oligonucleotide, 20 μL, Green.
7. Mutant Consensus dsDNA Oligonucleotide, 20 μL, Purple.
8. 10x Wash Buffer, 50 mL, Clear.
9. 2x Binding Buffer, 12 mL, Clear.
10. Primary Antibody Diluent, 12 mL, Clear.
11. 100x Protease and Phosphatase Inhibitors, 100 μL, Blue.
12. Nuclear Wash Buffer, 12 mL, Clear.
13. Cytoplasmic Extraction Buffer, 6 mL, Amber/
14. Nuclear Extraction Buffer, 6 mL, Amber.
15. Ready-to-Use Substrate, 12 mL, Brown.
16. Stop Solution, 12 mL, Clear.
17. Adhesive Plate Sealers, 4 Sheets.
18. Technical Manual, 1 Manual.

Storage

4°C/6 Months

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Genetic variation in the nuclear factor kappa B pathway in relation to susceptibility to rheumatoid arthritis

ANNALS OF THE RHEUMATIC DISEASES

Authors: Dieguez-Gonzalez, R.; Akar, S.; Calaza, M.; Perez-Pampin, E.; Costas, J.; Torres, M.; Vicario, J. L.; Velloso, M. L.; Navarro, F.; Narvaez, J.; Joven, B.; Herrero-Beaumont, G.; Gonzalez-Alvaro, I.; Fernandez-Gutierrez, B.; de la Serna, A. R.; Carreno, L.; Lopez-Longo, J.; Caliz, R.; Collado-Escobar, M. D.; Blanco, F. J.; Fernandez-Lopez, C.; Balsa, A.; Pascual-Salcedo, D.; Gomez-Reino, J. J.; Gonzalez, A.

Abstract

Objective: To examine genetic association between rheumatoid arthritis (RA) and known polymorphisms in core genes of the nuclear factor (NF)kappa B pathway, the major intracellular pathway in RA pathogenesis. Methods: Discovery and replication sample sets of Spanish patients with RA and controls were studied. A total of 181 single nucleotide polymorphisms ( SNPs) uniformly spaced along the genomic sequences of 17 core genes of the NFkB pathway (REL, RELA, RELB, NFKB1, NFKB2, NFKBIA, NFKBIB, NFKBIE, IKBKA, IKBKB, IKBKE, IKBKAP, KBRAS1, KBRAS2, MAP3K1, MAP3K14, TAX1BP1) were studied by mass spectrometry analysis complemented with 5'-nuclease fluorescence assays in the discovery set, 458 patients with RA and 657 controls. SNPs showing nominal significant differences were further investigated in the replication set of 1189 patients with RA and 1092 controls. Results: No clear reproducible association was found, although 12 SNPs in IKBKB, IKBKE and REL genes showed significant association in the discovery set. Interestingly, two of the SNPs in the IKBKE gene, weakly associated in the discovery phase, showed a trend to significant association in the replication phase. Pooling both sample sets together, the association with these two SNPs was significant. Conclusion: We did not find any major effect among the explored members of the NF-kappa B pathway in RA susceptibility. However, it is possible that variation in the IKBKE gene could have a small effect that requires replication in additional studies.

Epstein-Barr virus latent membrane protein (LMP1) induces specific NF kappa B complexes in human T-lymphoid cells

VIRUS RESEARCH

Authors: Chien, ML; Hammarskjold, ML

Abstract

The Epstein-Barr virus (EBV) latent membrane protein (LMP1) is believed to play a crucial role in oncogenesis mediated by this virus. We and others previously showed that LMP1 can induce NF kappa B activity in several non-lymphoid cells and B-lymphoid cell lines. Here we show that LMP1 is also able to efficiently induce NF kappa B in human T-lymphoid and monocytic cells. Specific NF kappa B complexes were detected in the nuclei of transfected Jurkat cells using gel mobility shift assays and Western blot analyses; Using antibodies, we demonstrated that these complexes contain NF kappa B subunits NFkB1, NFkB2, RelA and c-Rel. Our results also showed that the NF kappa B complexes induced by LMP1 are able to bind to the NF kappa B consensus sequence in the promoter of the interleukin-2 alpha receptor gene and induce expression from a minimal promoter linked to four tandem copies of this sequence. This suggests a possible mechanism by which LMP1 could induce T-cell activation and proliferation. (C) 2000 Elsevier Science B.V. All rights reserved.