The hybridomas are produced by fusion of BALB/c spleen cells with SP2/0-AG14 myeloma cells; this hybridoma is a non-producer; Plasma IL-6 activity was quantified as the ability to promote growth of the IL-6-dependent B-cell hybridoma; Cells are IL-6-dependent; confirmed as mouse with IEF of AST, PEP B and by species PCR. Cytogenetics: apparently murine chromosome morphology with extensive centric fusions. Viruses: ELISA: reverse transcriptase negative; PCR: SMRV
Application Notes
Bioassay for IL-6
Format
Frozen
Morphology
Round cells growing in suspension
Growth Properties
suspension
CultureMethod
Subculturing
Split ratio: 1: 2 to 1:3, every 2-3 days; seed out at 0.5 x 10^6 cells/ml; Doubling time: 50 hours; Harvest: maximal density of about 0.8 x 10^6 cells/ml.Add IL-6 every 1-2 days in the first week and cells have a high metabolic activity aciditying the culture medium
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