Intended Use
The Mouse Anti-Histone Total Ig ELISA Kit is an immunoassay suitable for quantifying or titering total antibody activity (IgG, IgA and IgM) specific for histone in serum or plasma. Other biological fluids, including tissue culture medium, may be validated for use.
Storage
The microtiter well plate and all other reagents, if unopened, are stable at 2-8°C until the expiration date printed on the box label. Stabilities of the working solutions are indicated under Reagent Preparation.
Discovery of KBD4466, a Selective TLR 7/8 Inhibitor, for the Treatment of Autoimmune Diseases
Yuan, Y., Wei, Y., Jia, Z., Chu, H., Kong, D., Lei, F., Ye, F., Qian, X., Zhang, J., Zhou, X., Zhu, X., Li, Z., Liang, X., & Chen, W.
Applications: ELISA
Reactive species: Mouse
"Abstract: The Toll-like receptor (TLR) family comprises a class of proteins that play a critical role in the innate immune system. Toll-like receptors TLR7 and TLR8 are transmembrane receptors that recognize single-stranded RNA. Aberrant activation of TLR7/8 is associated with the progression of certain autoimmune diseases, such as lupus. Therefore, highly selective inhibitors of TLR7/8 would be an effective therapeutic approach. Here, we have discovered a small molecule inhibitor, KBD4466 (19), which demonstrates excellent cellular activity, favorable pharmacokinetic properties, potent inhibition of cytokine production in mouse pharmacodynamic studies, and outstanding therapeutic efficacy in an SLE animal model. Furthermore, we have developed an efficient synthetic route to access a [3.1.0] bicyclic system featuring chiral contiguous quaternary carbon centers."
Article snippet: Plasma autoantibodies, including antihistone antibodies (Creative Diagnostics, DEIA120J), antiribosomal protein P antibodies (*), and anti-dsDNA antibodies (Creative Diagnostics, DEIA4488), were measured using the corresponding ELISA kits. 
Figure 1. Plasma levels of antihistone antibodies were quantified by ELISA.
COA
Publication 
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