Sample
serum, plasma, cell culture supernatants, tissue homogenate
Detection Method
Sandwich ELISA
Intended Use
For quantitative detection of Mouse Amot (Angiomotin) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
78-5000 pg/mL
Citations
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Sugihara-Mizuno, Y; Adachi, M; et al. Molecular characterization of angiomotin/JEAP family proteins: interaction with MUPP1/Patj and their endogenous properties. GENES TO CELLS 12:473-486(2007).
Holmgren, L; Ambrosino, E; et al. A DNA vaccine targeting angiomotin inhibits angiogenesis and suppresses tumor growth. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 103:9208-9213(2006).